Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi 515134, Andhra Pradesh, India.
Department of Physics, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi 515134, Andhra Pradesh, India.
J Microbiol Methods. 2022 Aug;199:106517. doi: 10.1016/j.mimet.2022.106517. Epub 2022 Jun 10.
The present study aimed at evaluating the extracellular synthesis of silver nanoparticles by soil fungus Aspergillus melleus SSS-10 for antibacterial and cytotoxic activity. In this study, the formation of silver nanoparticles (AgNPs) was estimated by the colour change in cell free extract from pale yellow to golden yellow after 24 h of the reaction. UV-Vis study showed the absorbance maxima at 410 nm. Tauc plot analysis revealed the band gap energy as 2.34 eV. Dynamic Light Scattering (DLS) data revealed polydisperse anisotropic silver nanoparticles with average hydrodynamic diameter of 92.006 nm. Zeta potential of - 19.6 mV provided evidence of stable silver nanoparticles. X-ray diffraction (XRD) analysis revealed four prominent Bragg peaks corresponding to (111), (200), (220) and (311) planes characteristic of silver (Ag) in FCC structural configuration. Average crystallite size was found to be 87.3 nm from Scherrer equation. Scanning Electron Microscope (SEM) analysis revealed irregular morphology of silver nanoparticles. EDS analysis displayed characteristic energy peaks of silver from 2.72 keV to 3.52 keV confirming the presence of silver nanoparticles. Biosynthesized AgNPs exhibited strong cytotoxic potential on MG-63 cells. AgNPs also showed antibacterial activity against both Staphylococcus aureus and Escherichia coli. In conclusion, this study provides a platform to explore the utility of fungal mediated silver nanoparticles synthesized for various pharmaceutical and cosmeceutical applications.
本研究旨在评估土壤真菌曲霉 SSS-10 细胞外合成银纳米粒子的抗菌和细胞毒性活性。在这项研究中，通过 24 小时反应后无细胞提取物从浅黄色变为金黄色来估计银纳米粒子（AgNPs）的形成。紫外-可见研究表明在 410nm 处有最大吸收。Tauc 图分析显示带隙能为 2.34eV。动态光散射（DLS）数据显示具有平均水动力直径为 92.006nm 的多分散各向异性银纳米粒子。Zeta 电位为-19.6mV 提供了稳定银纳米粒子的证据。X 射线衍射（XRD）分析显示四个突出的 Bragg 峰对应于银（Ag）的 FCC 结构构型中的（111）、（200）、（220）和（311）面。根据谢勒方程，平均晶粒尺寸为 87.3nm。扫描电子显微镜（SEM）分析显示银纳米粒子的不规则形态。EDS 分析显示银的特征能量峰从 2.72keV 到 3.52keV，证实了银纳米粒子的存在。生物合成的 AgNPs 对 MG-63 细胞表现出很强的细胞毒性。AgNPs 还对金黄色葡萄球菌和大肠杆菌表现出抗菌活性。总之，本研究为探索真菌介导的银纳米粒子在各种制药和化妆品应用中的应用提供了一个平台。
