Feil W, Wenzl E, Vattay P, Starlinger M, Sogukoglu T, Schiessel R
Gastroenterology. 1987 Jun;92(6):1973-86. doi: 10.1016/0016-5085(87)90632-9.
Epithelial repair after luminal acid exposure was studied in the rabbit duodenum in vivo and in vitro. Hydrochloric acid (200 mM, 30 min in vivo; 10 mM, 10 min in vitro) caused a uniform damage of the mucosa confined to the villi. After demarcation and detachment of the necrotic tissue the defect was bridged by the remaining viable epithelial cells, resulting in a reduction of villus height. This process of mucosal repair proceeded continuously, so that 9 h after acid damage only 33% of villi in the proximal duodenum and 41% in the distal duodenum remained not fully restored, at both luminal pH 7 and luminal pH 3. The difference is due to the higher acid susceptibility of the distal duodenum. In vitro, most of the mucosal surface was reconstituted 5 h after acid injury, at both luminal pH 7.4 and luminal pH 3. The potential difference declined and reversed after mucosal damage; restitution to original values was only observed at luminal pH 7 in vivo and luminal pH 7.4 in vitro.
在体内和体外对兔十二指肠腔内酸暴露后的上皮修复进行了研究。盐酸（体内200 mM，30分钟；体外10 mM，10分钟）导致局限于绒毛的黏膜均匀损伤。在坏死组织分界并脱离后，缺损由剩余的存活上皮细胞桥接，导致绒毛高度降低。这种黏膜修复过程持续进行，因此在酸损伤后9小时，无论腔内pH值为7还是pH值为3，十二指肠近端只有33%的绒毛和远端十二指肠41%的绒毛仍未完全恢复。差异是由于十二指肠远端对酸的敏感性较高。在体外，无论腔内pH值为7.4还是pH值为3，酸损伤后5小时大部分黏膜表面得以重建。黏膜损伤后电位差下降并反转；仅在体内腔内pH值为7和体外腔内pH值为7.4时观察到恢复到原始值。
