Black B A, Morris G P, Wallace J L
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;50(2):109-18. doi: 10.1007/BF02889895.
Rapid restitution of the gastric and intestinal epithelium after acute injury involves emigration of cells from the gastric glands and basal half of the intestinal villi. An intact basal lamina is prerequisite to the restitution process. The present study was performed to determine the effects of acid on the rat gastric and duodenal basal lamina. The basal lamina was denuded in vitro by ultrasonic vibration. The tissue was then immersed in 0.2 M mannitol (control) or in HCl (5-50 mM) for 10 min. Samples of the tissues were examined by transmission and scanning electron microscopy. Some samples were stained with ruthenium red to demonstrate glycosaminoglycans. The lower concentrations of acid (5 and 10 mM) had little or no effect on the structure of the basal lamina. However, exposure to 20 and 50 mM HCl caused extensive damage to the basal lamina and exposed the underlying connective tissue matrix of the lamina propria. Ruthenium red staining demonstrated differences in size and location of glycosaminoglycans within the basal laminae of stomach and intestine. Exposure to acid at concentrations of 20 or 50 mM caused total loss of ruthenium red staining in both intestinal and gastric basal laminae. Exposure to 10 mM acid resulted in loss of the outermost (luminal) layer of anionic sites from the gastric basal lamina. These studies demonstrate that brief exposure to acid, in concentrations which are necessary for the formation of hemorrhagic erosions in the stomach, caused damage to the basal lamina. This damage may impair epithelial restitution and thus account, in part, for the role of acid in ulcerogenesis.
急性损伤后胃和肠上皮的快速修复涉及胃腺和肠绒毛基部一半的细胞迁移。完整的基底膜是修复过程的前提条件。本研究旨在确定酸对大鼠胃和十二指肠基底膜的影响。通过超声振动在体外剥脱基底膜。然后将组织浸入0.2M甘露醇（对照）或HCl（5 - 50mM）中10分钟。通过透射和扫描电子显微镜检查组织样本。一些样本用钌红染色以显示糖胺聚糖。较低浓度的酸（5和10mM）对基底膜结构几乎没有影响或没有影响。然而，暴露于20和50mM HCl会对基底膜造成广泛损伤，并暴露固有层下面的结缔组织基质。钌红染色显示胃和肠基底膜内糖胺聚糖的大小和位置存在差异。暴露于20或50mM浓度的酸会导致肠和胃基底膜中的钌红染色完全丧失。暴露于10mM酸会导致胃基底膜最外层（腔面）阴离子位点丧失。这些研究表明，短暂暴露于酸（浓度为胃中形成出血性糜烂所必需的）会对基底膜造成损伤。这种损伤可能会损害上皮修复，从而部分解释酸在溃疡形成中的作用。
