超声处理对三七花多糖提取效率、特性和生物活性的影响。
Ultrasonic disruption effects on the extraction efficiency, characterization, and bioactivities of polysaccharides from Panax notoginseng flower.
机构信息
College of Chemistry, Changchun Normal University, Changchun 130032, China.
College of Chemistry, Changchun Normal University, Changchun 130032, China.
出版信息
Carbohydr Polym. 2022 Sep 1;291:119535. doi: 10.1016/j.carbpol.2022.119535. Epub 2022 Apr 27.
Ultrasonic disruption extraction (UDE) and heat reflux extraction (HRE) were used to extract polysaccharides (PNFPs) from Panax notoginseng flower, and the yield, physicochemical characteristics, structural characteristics, functional properties and biological activities were evaluated and compared. Results indicated that UDE changed the above properties of PNFPs. Compared with HRE (H-PNFPs), the yields, uronic acid contents, total phenolic content, total flavonoid content, water-solubility, water holding capacity, and oil holding capacity of the polysaccharides (U-PNFPs) extracted by UDE increased by 48.63%, 47.36%, 39.98%, 7.41%, 2.26%, 15.56%, and 1.34%, respectively. In comparison, the molecular weight and protein content decreased by 43.53% and 16.46%. Results demonstrate that UDE could effectively improve the physicochemical and functional properties, and bioactivities. These findings are helpful for better understanding the relationship between the physicochemical properties and bioactivities of U-PNFPs, especially U-PNFP-2 exhibited the most significant antioxidant activity, which can prevent oxidative stress, applications in food and pharmaceutical industries.
超声破碎提取 (UDE) 和热回流提取 (HRE) 被用于从三七花中提取多糖 (PNFPs)，并对其产率、理化特性、结构特性、功能特性和生物活性进行了评价和比较。结果表明，UDE 改变了 PNFPs 的上述性质。与 HRE (H-PNFPs) 相比，UDE 提取的多糖 (U-PNFPs) 的产率、糖醛酸含量、总酚含量、总黄酮含量、水溶性、持水能力和持油能力分别提高了 48.63%、47.36%、39.98%、7.41%、2.26%、15.56%和 1.34%，而分子量和蛋白质含量分别降低了 43.53%和 16.46%。结果表明，UDE 可以有效地提高多糖的理化和功能特性以及生物活性。这些发现有助于更好地理解 U-PNFPs 的理化性质和生物活性之间的关系，特别是 U-PNFP-2 表现出最显著的抗氧化活性，可预防氧化应激，在食品和制药行业有应用前景。
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