超声辅助 Fenton 降解对 进行水解得到的寡糖的提取、结构和抗氧化性能。
Extraction, Structural, and Antioxidant Properties of Oligosaccharides Hydrolyzed from by Ultrasonic-Assisted Fenton Degradation.
机构信息
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
出版信息
Int J Mol Sci. 2023 Feb 24;24(5):4506. doi: 10.3390/ijms24054506.
Plant polysaccharides exhibit many biological activities that are remarkably affected by molecular size and structures. This study aimed to investigate the degradation effect of ultrasonic-assisted Fenton reaction on the polysaccharide (PP). PP and its three degradation products (PP3, PP5, and PP7) were obtained from optimized hot water extraction and different Fenton reaction treatments, respectively. The results showed that the molecular weight (Mw) of the degraded fractions significantly decreased after treatment with the Fenton reaction. But the backbone characteristics and conformational structure were similar between PP and PP-degraded products, which was estimated by comparing monosaccharides composition, functional group signals in FT-IR spectra, X-ray differential patterns, and proton signals in H NMR. In addition, PP7, with an Mw of 5.89 kDa, exhibited stronger antioxidant activities in both the chemiluminescence-based and HHL5 cell-based methods. The results indicated that ultrasonic-assisted Fenton degradation might be used to improve the biological activities of natural polysaccharides by adjusting the molecular size.
植物多糖具有多种生物活性，其活性受分子大小和结构的显著影响。本研究旨在探讨超声辅助芬顿反应对多糖（PP）的降解效果。通过优化热水提取和不同芬顿反应处理，分别得到 PP 及其三种降解产物（PP3、PP5 和 PP7）。结果表明，芬顿反应处理后，降解产物的分子量（Mw）显著降低。但通过比较单糖组成、傅里叶变换红外光谱（FT-IR）中官能团信号、X 射线差示图谱和 H 核磁共振（H NMR）中的质子信号，推测 PP 与 PP 降解产物的骨架特征和构象结构相似。此外，Mw 为 5.89 kDa 的 PP7 在化学发光法和 HHL5 细胞法中均表现出较强的抗氧化活性。结果表明，通过调整分子大小，超声辅助芬顿降解可能用于提高天然多糖的生物活性。
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