AO Research Institute Davos, Davos, Switzerland; Department of Biomaterials Science and Technology, Faculty of Science and Technology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, the Netherlands.
Department of Biomaterials Science and Technology, Faculty of Science and Technology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, the Netherlands.
Carbohydr Polym. 2022 Sep 1;291:119550. doi: 10.1016/j.carbpol.2022.119550. Epub 2022 May 2.
Bacterial exopolysaccharides (EPS) are water-soluble polymers consisting of repeating sugar moieties that serve a wide range of functions for the bacterial species that produce them. Their functions include biofilm matrix constituent, nutrient retention, protection from environmental threats and even pathogenicity. EPS have also been exploited for use in various applications in the biomedical field: most notably as viscosupplements, drug delivery vehicles and in tissue engineering constructs. The use of EPS in bone tissue engineering has increased in recent years due to the wide range of compounds available, low cost, and ease of production on an industrial scale. This review discusses the extraction and purification methods employed to produce bacterial EPS. A particular focus is on bone-related tissue engineering applications where EPS is the primary active agent, or as a scaffold matrix, as well as a carrier for osteopromotive agents.
细菌胞外多糖 (EPS) 是由重复糖单位组成的水溶性聚合物，对产生它们的细菌种类具有广泛的功能。它们的功能包括生物膜基质成分、营养物质保留、抵御环境威胁甚至致病性。EPS 还被用于生物医学领域的各种应用：最著名的是作为黏弹性补充剂、药物输送载体和组织工程构建物。由于可用的化合物种类繁多、成本低且易于在工业规模上生产，近年来 EPS 在骨组织工程中的应用有所增加。本文综述了用于生产细菌 EPS 的提取和纯化方法。特别关注 EPS 是主要活性物质的骨相关组织工程应用，以及作为支架基质和成骨促进剂载体。
