School of Materials Science and Engineering, Key Laboratory of Polymeric Composite Materials and Functional Materials of Ministry of Education, GD Research Center for Functional Biomaterials Engineering and Technology, Sun Yat-sen University, Guangzhou 510275, China; The First Dongguan Affiliated Hospital of Guangdong Medical University, The Second Clinical Medical College, Guangdong Medical University, Dongguan 523808, China.
School of Materials Science and Engineering, Key Laboratory of Polymeric Composite Materials and Functional Materials of Ministry of Education, GD Research Center for Functional Biomaterials Engineering and Technology, Sun Yat-sen University, Guangzhou 510275, China.
Carbohydr Polym. 2022 Sep 1;291:119616. doi: 10.1016/j.carbpol.2022.119616. Epub 2022 May 14.
3D printing hydrogel is attractive to fabricate biomimetic scaffolds for skin repair. However, hydrogels that can be directly 3D printed with excellent mechanical properties are still limited. Here, a dual crosslinked hydrogel based on dynamic oxime crosslinking and hydrophobic interaction is developed for direct extrusion printing. We apply aminooxy terminated Pluronic F127 (AOP127) and oxidized dextran (ODex) as materials. At a lower temperature (ca. 16 °C), AOP127 and ODex form hydrogel with oxime binding for extrusion printing. At higher temperature (37 °C), the PPO segments of AOP127 physically associate, forming the second crosslinking to toughen the hydrogel. The hydrogel exhibits excellent thermosensitivity and self-healability. By 3D printing of AOP127-ODex-15% hydrogel, the obtained scaffolds show high toughness and excellent cytocompatibility. After coated with fibroin, the composite constructs showed adhesion and proliferation of skin cells. The present hydrogel is promising for fabrication of tough scaffold with natural polysaccharides and synthetic polymers.
3D 打印水凝胶在制备仿生皮肤修复支架方面具有吸引力。然而，能够具有优异机械性能的可直接 3D 打印的水凝胶仍然有限。在这里，开发了一种基于动态肟交联和疏水相互作用的双交联水凝胶，用于直接挤压打印。我们应用端氨基聚氧乙烯醚封端的泊洛沙姆 F127（AOP127）和氧化葡聚糖（ODex）作为材料。在较低的温度（约 16°C）下，AOP127 和 ODex 形成肟键结合的水凝胶，用于挤出打印。在较高的温度（37°C）下，AOP127 的 PPO 段物理缔合，形成第二交联以增强水凝胶的韧性。该水凝胶表现出优异的温敏性和自修复性。通过 AOP127-ODex-15%水凝胶的 3D 打印，获得的支架表现出高韧性和优异的细胞相容性。用丝素蛋白涂覆后，复合结构表现出皮肤细胞的黏附和增殖。这种水凝胶有望用于制备具有天然多糖和合成聚合物的坚韧支架。
