Parunyakul Kongphop, Srisuksai Krittika, Santativongchai Pitchaya, Pongchairerk Urai, Ampawong Sumate, Tulayakul Phitsanu, Fungfuang Wirasak
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Bio-Veterinary Sciences (International Program), Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Vet World. 2022 Apr;15(4):986-997. doi: 10.14202/vetworld.2022.986-997. Epub 2022 Apr 19.
Consumption of fatty acids (FA) can alter hepatic energy metabolism and mitochondrial function in the liver. Crocodile oil (CO) is rich in mono-and polyunsaturated FAs, which have natural anti-inflammatory and healing properties. In rat livers, we investigated the effect of CO on mitochondrial function for energy homeostasis.
Twenty-one male Sprague-Dawley rats were divided into three groups at random. Group 1 rats were given sterile water (RO), Group 2 rats were given CO (3% v/w), and Group 3 rats were given palm oil (PO) (3% v/w). For 7 weeks, rats were given sterile water, CO, and PO orally. The researchers looked at body weight, food intake, liver weight, energy intake, blood lipid profiles, and mitochondria-targeted metabolites in the liver. The liver's histopathology, mitochondrial architecture, and hydrolase domain containing 3 (HDHD3) protein expression in liver mitochondria were studied.
Body weight, liver weight, liver index, dietary energy intake, and serum lipid profiles were all unaffected by CO treatment. The CO group consumed significantly less food than the RO group. The CO group also had significantly higher levels of oxaloacetate and malate than the PO group. CO treatment significantly ameliorated hepatic steatosis, as evidenced by a greater decrease in the total surface area of lipid particles than PO treatment. CO administration preserved mitochondrial morphology in the liver by upregulating the energetic maintenance protein HDHD3. Furthermore, chemical-protein interactions revealed that HDHD3 was linked to the energy homeostatic pathway.
CO may benefit liver function by preserving hepatic mitochondrial architecture and increasing energy metabolic activity.
脂肪酸（FA）的摄入可改变肝脏的能量代谢和线粒体功能。鳄鱼油（CO）富含单不饱和脂肪酸和多不饱和脂肪酸，具有天然的抗炎和愈合特性。在大鼠肝脏中，我们研究了CO对能量稳态下线粒体功能的影响。
将21只雄性Sprague-Dawley大鼠随机分为三组。第1组大鼠给予无菌水（RO），第2组大鼠给予CO（3% v/w），第3组大鼠给予棕榈油（PO）（3% v/w）。连续7周，大鼠经口给予无菌水、CO和PO。研究人员观察了体重、食物摄入量、肝脏重量、能量摄入量、血脂谱以及肝脏中靶向线粒体的代谢产物。研究了肝脏的组织病理学、线粒体结构以及肝线粒体中含水解酶结构域3（HDHD3）蛋白的表达。
CO处理对体重、肝脏重量、肝脏指数、饮食能量摄入和血清脂质谱均无影响。CO组的食物摄入量明显低于RO组。CO组的草酰乙酸和苹果酸水平也显著高于PO组。CO处理显著改善了肝脂肪变性，脂质颗粒总表面积的减少幅度大于PO处理。CO给药通过上调能量维持蛋白HDHD3来维持肝脏中的线粒体形态。此外，化学-蛋白质相互作用表明HDHD3与能量稳态途径相关。
CO可能通过维持肝脏线粒体结构和增加能量代谢活性而有益于肝功能。
