Kasetsart University Research and Development Institute, Kasetsart University, Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand.
Department of Zoology, Faculty of Science, Kasetsart University, Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand.
Exp Anim. 2023 Nov 9;72(4):425-438. doi: 10.1538/expanim.23-0009. Epub 2023 Apr 7.
The liver is a key organ governing body energy metabolism. Dietary fats influence energy metabolism and mitochondrial functioning. Crocodile oil (CO) is rich in mono- and polyunsaturated fatty acids that contain natural anti-inflammatory and healing properties. Our study examined how CO affects the expressions of liver proteins involved in energy metabolism in rats. Twenty-one male Sprague Dawley rats were divided into three groups and underwent oral gavage with 3 ml/kg of sterile water (N group), CO (CO group), or palm oil (PO group) for 7 weeks. Body weight, energy intake, liver weight, liver indexes, blood lipid profiles, and liver-energy intermediates were measured. The liver proteome was analyzed using shotgun proteomics, and the functions and network interactions of several candidate proteins were predicted using the STITCH v.5.0 software. Body weights, energy intake, liver contents, and lipid profiles did not differ between the groups. However, hepatic oxaloacetate and malate levels were significantly higher in the CO group than in the PO group. Targeted proteomics reveals that 22 out of 1,790 unique proteins in the CO group were involved in energy-generating pathways, including the tricarboxylic acid cycle and oxidative phosphorylation (OXPHOS), and were correlated with the AMP-activated protein kinase signaling pathway. Cluster analysis of 59 differentially expressed proteins showed that OXPHOS-associated proteins were upregulated in the CO group and that three glycolytic metabolism-related proteins were downregulated in the CO group. CO may enhance hepatic energy metabolism by regulating the expressions of energy expenditure-related proteins.
肝脏是调节机体能量代谢的关键器官。膳食脂肪影响能量代谢和线粒体功能。鳄梨油（CO）富含单不饱和脂肪酸和多不饱和脂肪酸，具有天然的抗炎和愈合特性。本研究探讨了 CO 如何影响参与能量代谢的大鼠肝脏蛋白的表达。21 只雄性 Sprague Dawley 大鼠随机分为三组，分别给予 3ml/kg 的无菌水（N 组）、CO（CO 组）或棕榈油（PO 组）灌胃，持续 7 周。测量体重、能量摄入、肝重、肝指数、血脂谱和肝能量中间产物。采用 shotgun 蛋白质组学分析肝蛋白质组，使用 STITCH v.5.0 软件预测几种候选蛋白的功能和网络相互作用。各组间体重、能量摄入、肝含量和血脂谱无差异。然而，CO 组的草酰乙酸和苹果酸水平明显高于 PO 组。靶向蛋白质组学揭示 CO 组中有 22 种独特蛋白（1790 种中的 22 种）参与产能途径，包括三羧酸循环和氧化磷酸化（OXPHOS），并与 AMP 激活的蛋白激酶信号通路相关。59 种差异表达蛋白的聚类分析显示，CO 组中与 OXPHOS 相关的蛋白上调，CO 组中三种糖酵解代谢相关蛋白下调。CO 可能通过调节与能量消耗相关蛋白的表达来增强肝脏的能量代谢。
