Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5648-5653. doi: 10.1099/ijsem.0.004459. Epub 2020 Sep 15.
A novel actinobacterium, designated strain K10HN5, was isolated from a peat soil sample collected from Kantulee peat swamp forest, Surat Thani Province, Thailand and its taxonomic position was determined using a polyphasic approach. Strain K10HN5 contained -diaminopimelic acid, arabinose, galactose, glucose and ribose in its whole-cell hydrolysates. The predominant menaquinone was MK-8(H). The major fatty acids were iso-C, iso-C and iso-CH. Mycolic acids were not present. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine and phosphatidylinositol. The 16S rRNA gene sequence analysis indicated that it was closely related to DSM 45300 (97.9 %) and JCM 11839 (97.9 %). Strain K10HN5 exhibited low average nucleotide identity and digital DNA-DNA hybridization values with DSM 45300 (82.6, 28.7 %) and JCM11839 (76.3, 22.2 %). The DNA G+C content of strain K10HN5 was 72.4 mol%. Based on polyphasic data, strain K10HN5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K10HN5 (=TBRC 10048=NBRC 113897).
一株新的放线菌，命名为 K10HN5 菌株，从泰国苏拉特他尼省 Kantulee 泥炭沼泽森林采集的泥炭土壤样本中分离得到，其分类地位采用多相方法确定。K10HN5 菌株的全细胞水解物含有 -二氨基庚二酸、阿拉伯糖、半乳糖、葡萄糖和核糖。主要的类异戊二烯醌为 MK-8(H)。主要脂肪酸为 iso-C15:0、iso-C16:0 和 iso-C17:0。不存在分枝菌酸。极性脂谱由双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂甲醚乙醇胺、羟基磷脂酰乙醇胺、羟基磷脂甲醚乙醇胺和磷脂酰肌醇组成。16S rRNA 基因序列分析表明，它与 DSM 45300（97.9%）和 JCM 11839（97.9%）密切相关。K10HN5 菌株与 DSM 45300（82.6%，28.7%）和 JCM11839（76.3%，22.2%）的平均核苷酸同一性和数字 DNA-DNA 杂交值较低。K10HN5 菌株的 DNA G+C 含量为 72.4 mol%。基于多相数据，K10HN5 菌株代表了属的一个新种，因此建议使用新种名。模式菌株为 K10HN5（=TBRC 10048=NBRC 113897）。
