对谷子（Setaria italica [L.] P. Beauv）组蛋白乙酰转移酶基因家族及其对生物和非生物胁迫反应的全基因组研究。
Genome-wide investigation of histone acetyltransferase gene family and its responses to biotic and abiotic stress in foxtail millet (Setaria italica [L.] P. Beauv).
机构信息
State Key Laboratory of Sustainable Dryland Agriculture, Shanxi Agricultural University, 030031, Taiyuan, China.
College of Agricultural, Shanxi Agricultural University, 030801, Jinzhong, China.
出版信息
BMC Plant Biol. 2022 Jun 14;22(1):292. doi: 10.1186/s12870-022-03676-9.
BACKGROUND
Modification of histone acetylation is a ubiquitous and reversible process in eukaryotes and prokaryotes and plays crucial roles in the regulation of gene expression during plant development and stress responses. Histone acetylation is co-regulated by histone acetyltransferase (HAT) and histone deacetylase (HDAC). HAT plays an essential regulatory role in various growth and development processes by modifying the chromatin structure through interactions with other histone modifications and transcription factors in eukaryotic cells, affecting the transcription of genes. Comprehensive analyses of HAT genes have been performed in Arabidopsis thaliana and Oryza sativa. However, little information is available on the HAT genes in foxtail millet (Setaria italica [L.] P. Beauv).
RESULTS
In this study, 24 HAT genes (SiHATs) were identified and divided into four groups with conserved gene structures via motif composition analysis. Phylogenetic analysis of the genes was performed to predict functional similarities between Arabidopsis thaliana, Oryza sativa, and foxtail millet; 19 and 2 orthologous gene pairs were individually identified. Moreover, all identified HAT gene pairs likely underwent purified selection based on their non-synonymous/synonymous nucleotide substitutions. Using published transcriptome data, we found that SiHAT genes were preferentially expressed in some tissues and organs. Stress responses were also examined, and data showed that SiHAT gene transcription was influenced by drought, salt, low nitrogen, and low phosphorus stress, and that the expression of four SiHATs was altered as a result of infection by Sclerospora graminicola.
CONCLUSIONS
Results indicated that histone acetylation may play an important role in plant growth and development and stress adaptations. These findings suggest that SiHATs play specific roles in the response to abiotic stress and viral infection. This study lays a foundation for further analysis of the biological functions of SiHATs in foxtail millet.
背景
组蛋白乙酰化修饰是真核生物和原核生物中普遍存在且可逆转的过程，在植物发育和应激响应过程中基因表达的调控中发挥着关键作用。组蛋白乙酰化由组蛋白乙酰转移酶（HAT）和组蛋白去乙酰化酶（HDAC）共同调控。在真核细胞中，HAT 通过与其他组蛋白修饰和转录因子相互作用来修饰染色质结构，从而在各种生长和发育过程中发挥重要的调节作用，影响基因的转录。在拟南芥和水稻中已经对 HAT 基因进行了全面分析。然而，关于谷子（Setaria italica [L.] P. Beauv）中的 HAT 基因知之甚少。
结果
本研究通过基序组成分析，从谷子中鉴定出 24 个 HAT 基因（SiHATs），并将其分为四个亚组，具有保守的基因结构。通过对基因的系统进化分析，预测了拟南芥、水稻和谷子之间的功能相似性；分别鉴定出 19 对和 2 对同源基因对。此外，所有鉴定出的 HAT 基因对都可能基于非同义/同义核苷酸替换经历了纯化选择。利用已发表的转录组数据，我们发现 SiHAT 基因在一些组织和器官中优先表达。还对胁迫响应进行了研究，结果表明 SiHAT 基因的转录受到干旱、盐、低氮和低磷胁迫的影响，并且由于感染腥黑穗病菌，四个 SiHAT 的表达发生了改变。
结论
结果表明，组蛋白乙酰化可能在植物生长发育和应激适应中发挥重要作用。这些发现表明 SiHATs 在非生物胁迫和病毒感染的响应中发挥特定作用。本研究为进一步分析谷子中 SiHATs 的生物学功能奠定了基础。
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