Rahman Md Asabur, Sultana Nasrin, Ayman Ummay, Bhakta Sonali, Afrose Marzia, Afrin Marya, Haque Ziaul
Department of Anatomy and Histology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Babugonj, Barisal 8210, Bangladesh.
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Saudi J Biol Sci. 2022 Jan;29(1):175-182. doi: 10.1016/j.sjbs.2021.08.075. Epub 2021 Aug 27.
Formalin is a widely used fixative but there is potential public health risks to exposure. Besides, alcoholic fixation is advantageous over formalin fixation because of faster fixation, optimal preservation and safer workplace environment. Following fixation by EMA and 10% neutral buffered formalin (NBF), we analyzed the tissue morphology, antigenic stability, DNA and RNA quantity with quality (OD value). The findings of EMA fixing on both the tissue morphology and molecular characterization, were satisfactory. Specially, EMA was faster in penetration of tissues than NBF, fixed ideally as early as 8 h of fixation whereas improper fixation was evident for NBF. In Hematoxylin and Eosin (H & E) staining, better cellular details with stronger affinity for staining were observed. In immunohistochemistry, better antigenic stability was reported for EMA-fixed tissues. The nucleic acid analysis revealed that total genomic DNA and RNA yield from EMA fixed tissues were significantly higher () with superior quality than NBF fixed tissues. Our results suggest that EMA could be a potential alternative to NBF for fixation and preservation of tissues. These data provide new insights into an option for a safer working environment to support study and research.
福尔马林是一种广泛使用的固定剂，但接触福尔马林存在潜在的公共健康风险。此外，酒精固定优于福尔马林固定，因为其固定速度更快、保存效果最佳且工作场所环境更安全。在用EMA和10%中性缓冲福尔马林（NBF）固定后，我们分析了组织形态、抗原稳定性、DNA和RNA的数量与质量（OD值）。EMA固定在组织形态和分子特征方面的结果均令人满意。特别是，EMA穿透组织的速度比NBF快，早在固定8小时就达到了理想的固定效果，而NBF则明显存在固定不当的情况。在苏木精和伊红（H&E）染色中，观察到细胞细节更好，染色亲和力更强。在免疫组织化学中，EMA固定的组织具有更好的抗原稳定性。核酸分析表明，EMA固定组织的总基因组DNA和RNA产量显著更高（），且质量优于NBF固定的组织。我们的结果表明，EMA可能是NBF用于组织固定和保存的潜在替代品。这些数据为支持研究和调查的更安全工作环境选项提供了新的见解。
