Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto, Japan.
Department of Biology, College of Natural Sciences, Can Tho University, Can Tho, Vietnam.
Curr Microbiol. 2022 Jun 15;79(8):221. doi: 10.1007/s00284-022-02907-4.
Vibrio parahaemolyticus is a bacterial pathogen in marine aquaculture systems and a major cause of food-borne illnesses worldwide. In the present study, Vibrio phage KIT05 was isolated from water collected from a shrimp farm in the Mekong Delta, Vietnam. It was characterized based on its morphology, growth curve, lytic properties, and genome sequence. Under the electron microscope, KIT05 particles had an icosahedral head with a diameter of 62.3 nm and a short tail of 24.1 nm. The one-step growth curve of KIT05 showed that its latency time was approximately 40 min and burst size was 18 plaque-forming units/cell. The genome of KIT05 comprises 50,628 bp with a GC content of 41.63%. It contains 60 open reading frames that are encoded within both strands and four tRNAs. The presence of direct terminal repeats of 130 bp at both ends of the KIT05 DNA was determined. According to phage morphology, genomic organization, and phylogeny analysis, Vibrio phage KIT05 was classified into the family Podoviridae. The genome annotation revealed that KIT05 had no virulent or lysogenic genes. This study may help identify a novel candidate for developing biocontrol agents for Vibrio parahaemolyticus.
副溶血弧菌是海洋水产养殖系统中的一种细菌病原体，也是全球食源性疾病的主要原因。本研究从越南湄公河三角洲的一个虾养殖场采集的水样中分离到了副溶血弧菌噬菌体 KIT05。根据其形态、生长曲线、裂解特性和基因组序列对其进行了表征。在电子显微镜下，KIT05 颗粒具有一个直径为 62.3nm 的二十面体头部和一个 24.1nm 的短尾。KIT05 的一步生长曲线表明，其潜伏期约为 40 分钟，爆发量为 18 个噬菌斑形成单位/细胞。KIT05 的基因组由 50628bp 组成，GC 含量为 41.63%。它包含 60 个开放阅读框，编码在两条链上，还有四个 tRNA。确定了 KIT05 DNA 两端 130bp 的直接末端重复序列。根据噬菌体形态、基因组组织和系统发育分析，副溶血弧菌噬菌体 KIT05 被归类为肌尾噬菌体科。基因组注释表明，KIT05 没有毒力或溶原基因。本研究可能有助于确定用于防治副溶血弧菌的新型生物防治剂候选物。
