Greenwald G I, Ganz T
Infect Immun. 1987 Jun;55(6):1365-8. doi: 10.1128/iai.55.6.1365-1368.1987.
An acid extract of human neutrophil granules was fractionated on a Sephadex G-100 column and tested for microbicidal activity against Acinetobacter calcoaceticus HO-1 as described previously (M.C. Modrzakowski and C. M. Paranavitana, Infect. Immun. 32:668-674, 1981). The low-molecular-weight protein fraction, peak D, accounted for about 30% of the protein and nearly all of the activity of the crude extract against strain HO-1. Peak D protein proved to be a mixture of the three human defensin peptides HNP1, HNP2, and HNP3. Purified defensins reproduced the microbicidal activity of peak D. The data suggest that defensins could play a major role in the killing of A. calcoaceticus by human neutrophils.
人中性粒细胞颗粒的酸性提取物在Sephadex G - 100柱上进行分级分离，并按照先前描述的方法（M.C.莫德扎科夫斯基和C.M.帕拉纳维塔纳，《感染与免疫》32:668 - 674，1981年）测试其对醋酸钙不动杆菌HO - 1的杀菌活性。低分子量蛋白质级分，即峰D，约占蛋白质的30%，并且几乎占粗提物对HO - 1菌株所有的活性。峰D蛋白被证明是三种人防御素肽HNP1、HNP2和HNP3的混合物。纯化的防御素重现了峰D的杀菌活性。数据表明，防御素可能在人中性粒细胞杀灭醋酸钙不动杆菌中起主要作用。
