Wang Mingyue, Miao Zhichao, Cen Hong, He Jiaxu, Wei Changyuan
Department of Hematology/Oncology, Guangxi Medical University Cancer Hospital, Nanning, China.
Department of Hematology, Nanxishan Hospital of Guangxi Zhuang Autonomous region, Guilin, China.
Transl Cancer Res. 2022 May;11(5):1362-1371. doi: 10.21037/tcr-22-1087.
Long non-coding RNA (LncRNA) play roles in different diseases, LncRNA is differentially expressed in diffuse large B cell lines with varying degrees of resistance to rituximab.
In the GEO database (GSE159852), we found that CHROMR (cholesterol induced regulator of metabolism RNA) may be differentially expressed in different rituximab-resistant diffuse large B lymphoma cell lines. We also verified the expression level in cell lines and verified the role of CHROMR in acquiring cell drug resistance through various biological function experiments. We predict that there may be a potential regulatory mechanism for CHROMR and validated it.
We found that CHROMR was differentially expressed in different rituximab-resistant cell lines. When the rituximab-sensitive cell line SU_DHL_4 was stimulated by rituximab, flow experiments demonstrated that overexpression of CHROMR could reduce the level of cell apoptosis and the proportion of arrested cells in the G2/M phase of the cell cycle. cck8 experiments demonstrated that overexpression of CHROMR increased cell proliferation. Western Blot (WB) experiments confirmed that overexpression of CHROMR reduced the expression of apoptosis-related proteins. Dual-luciferase and recovery experiments suggested that CHROMR acted through the CHROMR/hsa-miR-1299/CNNM1 pathway.
lncRNA CHROMR promotes the expression of the gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells.
长链非编码RNA（LncRNA）在不同疾病中发挥作用，LncRNA在对利妥昔单抗具有不同程度抗性的弥漫性大B细胞系中差异表达。
在GEO数据库（GSE159852）中，我们发现CHROMR（胆固醇诱导的代谢调节RNA）可能在不同的利妥昔单抗耐药弥漫性大B淋巴瘤细胞系中差异表达。我们还验证了其在细胞系中的表达水平，并通过各种生物学功能实验验证了CHROMR在获得细胞耐药性中的作用。我们预测CHROMR可能存在潜在的调控机制并进行了验证。
我们发现CHROMR在不同的利妥昔单抗耐药细胞系中差异表达。当利妥昔单抗敏感细胞系SU_DHL_4受到利妥昔单抗刺激时，流式实验表明CHROMR的过表达可降低细胞凋亡水平以及细胞周期G2/M期的停滞细胞比例。cck8实验表明CHROMR的过表达增加了细胞增殖。蛋白质免疫印迹（WB）实验证实CHROMR的过表达降低了凋亡相关蛋白的表达。双荧光素酶和回复实验表明CHROMR通过CHROMR/hsa-miR-1299/CNNM1途径发挥作用。
lncRNA CHROMR通过吸附hsa-miR-1299促进基因表达，从而在弥漫性大B淋巴瘤细胞中获得耐药性。
