Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Center for Applied Malaria Research and Evaluation, Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana, USA.
J Infect Dis. 2022 Oct 17;226(8):1461-1469. doi: 10.1093/infdis/jiac245.
Serological data can provide estimates of human exposure to both malaria vector and parasite based on antibody responses. A multiplex bead-based assay was developed to simultaneously detect IgG to Anopheles albimanus salivary gland extract (SGE) and 23 Plasmodium falciparum antigens among 4185 participants enrolled in Artibonite department, Haiti in 2017. Logistic regression adjusted for participant- and site-level covariates and found children under 5 years and 6-15 years old had 3.7- and 5.4-fold increase in odds, respectively, of high anti-SGE IgG compared to participants >15 years. Seropositivity to P. falciparum CSP, Rh2_2030, and SEA-1 antigens was significantly associated with high IgG response against SGE, and participant enrolment at elevations under 200 m was associated with higher anti-SGE IgG levels. The ability to approximate population exposure to malaria vectors through SGE serology data is very dependent by age categories, and SGE antigens can be easily integrated into a multiplex serological assay.
血清学数据可根据抗体反应，估算人类对疟蚊和疟原虫的接触情况。2017 年，在海地阿蒂博尼特省招募了 4185 名参与者，开发了一种基于多重微珠的检测方法，用于同时检测针对致倦库蚊唾液腺提取物（SGE）和 23 种恶性疟原虫抗原的 IgG。经参与者和地点协变量调整的逻辑回归发现，与 15 岁以上的参与者相比，5 岁以下和 6-15 岁的儿童对 SGE 的 IgG 抗体具有 3.7 倍和 5.4 倍的高抗体几率增加。对恶性疟原虫 CSP、Rh2_2030 和 SEA-1 抗原的血清阳性与对 SGE 的高 IgG 反应呈显著相关，参与者在海拔低于 200 米的地方登记与较高的 SGE IgG 水平相关。通过 SGE 血清学数据来估计人群对疟蚊的接触情况非常依赖年龄类别，并且 SGE 抗原可以很容易地整合到多重血清学检测中。
