Department of Periodontology, Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, People's Republic of China.
The Affiliated Hospital of Stomatology, Zhejiang University School of Medicine and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, People's Republic of China.
J Clin Periodontol. 2022 Sep;49(9):922-931. doi: 10.1111/jcpe.13683. Epub 2022 Jun 30.
To explore the immunological defensive effects of platelets on periodontal pathogens in the gingival crevicular fluid (GCF).
GCF samples were collected from 20 patients with periodontitis and 10 healthy controls. Platelets in the GCF were detected by immunocytochemistry and immunofluorescence. Isolated platelets from healthy volunteers were co-cultured with Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn). The interactions between platelets and periodontal pathogens were observed by transmission and scanning electron microscopy. The isolated platelets plus neutrophils were co-cultured with Pg or Fn, and the formation of neutrophil extracellular traps (NETs) was evaluated by staining with Sytox Green.
The platelet level in the GCF was higher in patients with periodontitis than in healthy controls. Platelets interacted with bacteria and neutrophils in the GCF. In vitro, platelets recruited and engulfed periodontal pathogens. In response to periodontal pathogens, neutrophils released web chromatin, and platelets promoted the formation of intensive NETs.
Platelets, migrating to the gingival sulcus, may exert direct antibacterial effects or assist neutrophils.
探讨龈沟液（GCF）中血小板对牙周病原菌的免疫防御作用。
采集 20 例牙周炎患者和 10 例健康对照者的 GCF 样本。通过免疫细胞化学和免疫荧光法检测 GCF 中的血小板。从健康志愿者中分离血小板，与牙龈卟啉单胞菌（Pg）和具核梭杆菌（Fn）共培养。通过透射和扫描电子显微镜观察血小板与牙周病原菌之间的相互作用。分离的血小板加中性粒细胞与 Pg 或 Fn 共培养，并用 Sytox Green 染色评估中性粒细胞胞外诱捕网（NETs）的形成。
牙周炎患者的 GCF 中血小板水平高于健康对照组。血小板与 GCF 中的细菌和中性粒细胞相互作用。在体外，血小板募集并吞噬牙周病原菌。对牙周病原菌的反应中，中性粒细胞释放网状染色质，血小板促进密集型 NETs 的形成。
迁移到牙龈沟的血小板可能发挥直接的抗菌作用或辅助中性粒细胞。
