牙周炎患者龈沟液中牙周致病菌与血小板的相互作用。

Interactions of periodontal pathogens with platelets in the gingival crevicular fluid of patients with periodontitis.

机构信息

Department of Periodontology, Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, People's Republic of China.

The Affiliated Hospital of Stomatology, Zhejiang University School of Medicine and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, People's Republic of China.

出版信息

J Clin Periodontol. 2022 Sep;49(9):922-931. doi: 10.1111/jcpe.13683. Epub 2022 Jun 30.

DOI:10.1111/jcpe.13683

PMID:35713232

Abstract

AIM

To explore the immunological defensive effects of platelets on periodontal pathogens in the gingival crevicular fluid (GCF).

MATERIALS AND METHODS

GCF samples were collected from 20 patients with periodontitis and 10 healthy controls. Platelets in the GCF were detected by immunocytochemistry and immunofluorescence. Isolated platelets from healthy volunteers were co-cultured with Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn). The interactions between platelets and periodontal pathogens were observed by transmission and scanning electron microscopy. The isolated platelets plus neutrophils were co-cultured with Pg or Fn, and the formation of neutrophil extracellular traps (NETs) was evaluated by staining with Sytox Green.

RESULTS

The platelet level in the GCF was higher in patients with periodontitis than in healthy controls. Platelets interacted with bacteria and neutrophils in the GCF. In vitro, platelets recruited and engulfed periodontal pathogens. In response to periodontal pathogens, neutrophils released web chromatin, and platelets promoted the formation of intensive NETs.

CONCLUSIONS

Platelets, migrating to the gingival sulcus, may exert direct antibacterial effects or assist neutrophils.

摘要

目的

探讨龈沟液（GCF）中血小板对牙周病原菌的免疫防御作用。

材料和方法

采集 20 例牙周炎患者和 10 例健康对照者的 GCF 样本。通过免疫细胞化学和免疫荧光法检测 GCF 中的血小板。从健康志愿者中分离血小板，与牙龈卟啉单胞菌（Pg）和具核梭杆菌（Fn）共培养。通过透射和扫描电子显微镜观察血小板与牙周病原菌之间的相互作用。分离的血小板加中性粒细胞与 Pg 或 Fn 共培养，并用 Sytox Green 染色评估中性粒细胞胞外诱捕网（NETs）的形成。