Insect Bioresource Laboratory, Animal Bioresources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Govt. of India, Takyelpat, Imphal 795001, Manipur, India; School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar 751024, Odisha, India.
School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar 751024, Odisha, India.
Pestic Biochem Physiol. 2022 Jun;184:105116. doi: 10.1016/j.pestbp.2022.105116. Epub 2022 May 10.
For the well-being of human health as well as ecological concerns and the development of insect resistance to conventional chemical insecticides, efforts have increased worldwide, to find eco-friendly, effective and safer insect control agents which are of natural origin. A bioactive biofumigant molecule named dihydro-p-coumaric acid was isolated and characterized from the leaves of Tithonia diversifolia Hemsl. A. Gray following laboratory bioassays against the rice weevil, Sitophilus oryzae L (Coleoptera: Curculionidae); the lesser grain borer, Rhyzopertha dominica F (Coleoptera: Bostrichidae) and the rust-red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). The isolated compound acted as a fumigant, toxic to adults of stored grain insect pests with LC values of 17.86, and 11.49 μg/L (S. oryzae), 19.80 and 10.29 μg/L (R. dominica) and 24.41 and 17.80 μg/L air (T. casatneum) respectively. Further, in vivo data reveal that the percentage of inhibition of acetyl cholinesterase (AChE) was dose-dependent and in vitro results showed potent AChE inhibitor. The isolated compound acts as an efficient biofumigant against the stored grain insect pests and has no adverse effect on seed germination. From this study, we assume that the isolated biofumigant molecule has the ability for used in IPM programs for stored-grain pests because of its biofumigant activity.
为了人类健康、生态关切以及昆虫对传统化学杀虫剂抗药性的发展，全世界都在努力寻找环保、有效和更安全的天然来源的昆虫控制剂。从黄桐属植物（Tithonia diversifolia Hemsl.）的叶子中分离并鉴定出一种具有生物活性的生物熏蒸分子，名为二氢对香豆酸。该分子在实验室对水稻象鼻虫（Sitophilus oryzae L）（鞘翅目：象甲科）、米象（Rhyzopertha dominica F）（鞘翅目：长蠹科）和赤拟谷盗（Tribolium castaneum Herbst）（鞘翅目：拟步甲科）进行生物测定后被发现具有熏蒸活性，对储粮害虫成虫具有毒性，LC 值分别为 17.86 和 11.49μg/L（S. oryzae）、19.80 和 10.29μg/L（R. dominica）和 24.41 和 17.80μg/L 空气（T. casatneum）。此外，体内数据显示乙酰胆碱酯酶（AChE）的抑制率呈剂量依赖性，体外结果表明该化合物是一种有效的 AChE 抑制剂。该分离化合物是一种有效的储粮害虫生物熏蒸剂，对种子发芽没有不良影响。从这项研究中，我们假设分离出的生物熏蒸分子具有在 IPM 计划中用于防治储粮害虫的能力，因为它具有生物熏蒸活性。
