Singh Kabrambam D, Koijam Arunkumar S, Bharali Rupjyoti, Rajashekar Yallappa
Insect Bioresource Laboratory, Animal Bioresources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal, Manipur, India.
Department of Biotechnology, Gauhati University, Guwahati, Assam, India.
Front Plant Sci. 2023 Feb 20;14:1135946. doi: 10.3389/fpls.2023.1135946. eCollection 2023.
The Last four decades have witnessed the banning of several synthetic insecticides mainly due to the development of resistance to the target pests and due to hazardous effects on humans and the environment. Hence, the development of a potent insecticide with biodegradable and eco-friendly nature is the need of the hour. In the present study, the fumigant property, and biochemical effects of L. (Dilleniaceae) were studied against three coleopterans stored-products insects. The bioactive enriched fraction (sub-fraction-III) was isolated from ethyl acetate extracts of leaves and found toxic to rice weevil, (L.) (Coleoptera); lesser grain borer (L.) (Coleoptera) and red flour beetle, (Herbst.) (Coleoptera) with the LC values of 101.887, 189.908 and 115.1 µg/L respectively after 24 h exposure. The enriched fraction was found to inhibit the function of acetylcholinesterase (AChE) enzyme when tested against , and with LC value of 88.57 µg/ml, 97.07 µg/ml, and 66.31 µg/ml respectively, in condition. It was also found that the enriched fraction caused a significant oxidative imbalance in the antioxidative enzyme system such as superoxide dismutase, catalase, DPPH (2,2-diphenyl-1-picrylhydrazyl), and glutathione-S-transferase (GST). GCMS analysis of the enriched fraction indicates three major compounds namely, 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one, 1,2-Benzisothiazol-3(2H)-one, and Benzothiazole, 2-(2-hydroxyethylthio)-. Finally, we concluded that the enriched fraction of has insecticidal properties and the toxicity may be due to the inhibition of the AChE enzyme in association with oxidative imbalance created on the insect's antioxidant enzyme systems.
在过去的四十年里，几种合成杀虫剂已被禁用，主要原因是目标害虫产生了抗药性，以及对人类和环境的有害影响。因此，开发一种具有可生物降解和生态友好特性的高效杀虫剂已成为当务之急。在本研究中，对来自五桠果科的某植物的熏蒸特性及其对三种鞘翅目储粮害虫的生化影响进行了研究。从该植物叶子的乙酸乙酯提取物中分离出生物活性富集级分（亚级分III），发现其对米象（鞘翅目）、谷蠹（鞘翅目）和赤拟谷盗（鞘翅目）有毒，暴露24小时后，其LC值分别为101.887、189.908和115.1微克/升。在特定条件下进行测试时，发现该富集级分对米象、谷蠹和赤拟谷盗具有抑制乙酰胆碱酯酶（AChE）功能的作用，其LC值分别为88.57微克/毫升、97.07微克/毫升和66.31微克/毫升。还发现该富集级分在抗氧化酶系统（如超氧化物歧化酶、过氧化氢酶、DPPH（2,2-二苯基-1-苦基肼）和谷胱甘肽-S-转移酶（GST））中引起了显著的氧化失衡。对该富集级分的GCMS分析表明存在三种主要化合物，即6-羟基-4,4,7a-三甲基-5,6,7,7a-四氢苯并呋喃-2(4H)-酮、1,2-苯并异噻唑-3(2H)-酮和2-(2-羟乙基硫代)苯并噻唑。最后，我们得出结论，该植物的富集级分具有杀虫特性，其毒性可能是由于抑制了AChE酶，并伴随着昆虫抗氧化酶系统产生的氧化失衡。
