Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
J Nat Prod. 2022 Jul 22;85(7):1715-1722. doi: 10.1021/acs.jnatprod.2c00174. Epub 2022 Jun 17.
Zelkovamycins F and G ( and ), two new natural cyclic octapeptides possessing the unprecedented nonproteinogenic amino acid residues l-α-methyl-threonine and l-α-methyl--threonine, respectively, along with four new analogues, zelkovamycins H-K (-), were identified from the endophytic sp. CPCC 204717. Their structures were elucidated by detailed analysis of NMR and HRESIMS/MS spectroscopic data. The configurations of amino acid residues were determined by Marfey's analysis combined with NMR calculations. Compounds , , and showed potent antibacterial activity against methicillin-resistant and . The structure-activity relationship study revealed that the 2-methyl-3-oxobutyrine and sarcosine residues played important roles in their antibacterial activities. Zelkovamycin () and zelkovamycin E () exhibited significant antiviral activity against the hepatitis C virus.
泽兰霉素 F 和 G（以及），两种新的天然环状八肽，分别含有前所未有的非蛋白氨基酸残基 l-α-甲基-苏氨酸和 l-α-甲基-β-苏氨酸，以及四个新的类似物，泽兰霉素 H-K（-），从内生菌 sp. CPCC 204717 中鉴定出来。通过详细分析 NMR 和 HRESIMS/MS 光谱数据阐明了它们的结构。氨基酸残基的构型通过 Marfey 分析结合 NMR 计算来确定。化合物 、 和 对耐甲氧西林的 和 表现出强烈的抗菌活性。构效关系研究表明，2-甲基-3-氧代丁酸和肌氨酸残基在其抗菌活性中发挥重要作用。泽兰霉素（）和泽兰霉素 E（）对丙型肝炎病毒表现出显著的抗病毒活性。
