Department of Woman, Child and General and Specialist Surgery, University of Campania "Luigi Vanvitelli", Naples, Italy.
Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Pediatr Res. 2023 Feb;93(3):520-525. doi: 10.1038/s41390-022-02109-5. Epub 2022 Jun 18.
The Cannabinoid Receptor type 2 (CB2) is involved in inflammation and immune cell modulation. In previous studies, we demonstrated the association between the CNR2 rs35761398 polymorphism and the risk for pediatric inflammatory bowel disease (IBD). In this study, we analyzed the intestinal biopsies from Crohn disease (CD) and ulcerative colitis (UC) pediatric patients at the diagnosis to evaluate the expression of CB2 and several factors associated with IBD inflammatory pathways.
We enrolled five patients with CD, five with UC, and five controls (CTR). We analyzed ileum and rectum biopsies from patients of each group evaluating the expression of CB2, Toll-like receptor 4, interleukin-6, and interleukin-1β by western blot and immunofluorescence.
Western blot analysis showed a significant increase of CB2 in the CD ileum and in the UC rectum biopsies and an increase of TLR4 in the UC rectum. We also observed a significant over-expression of the IL-6 in UC rectum. The immunofluorescence analysis confirmed western blot data, showing also a T-lymphocytes infiltration colocalized with CB2 expression in the CD ileum and UC rectum.
Our results show an upregulation of CB2 in pediatric IBD, which might have implications for drug discovery.
The Cannabinoid Receptor type 2 (CB2) is involved in the inflammation and modulation of the immune response in pediatric inflammatory bowel disease (IBD). CB2 receptor is more expressed in the inflamed intestine of pediatric IBD patients. CB2 could be used as a potential therapeutic target to reduce IBD-related inflammatory state in childhood.
大麻素受体 2 型（CB2）参与炎症和免疫细胞调节。在之前的研究中，我们证明了 CNR2 rs35761398 多态性与儿科炎症性肠病（IBD）的风险之间存在关联。在这项研究中，我们分析了克罗恩病（CD）和溃疡性结肠炎（UC）儿科患者的诊断时的肠道活检组织，以评估 CB2 的表达以及与 IBD 炎症途径相关的几种因素。
我们纳入了 5 例 CD 患者、5 例 UC 患者和 5 例对照（CTR）。我们分析了每个组患者的回肠和直肠活检组织，通过 Western blot 和免疫荧光法评估 CB2、Toll 样受体 4、白细胞介素 6 和白细胞介素 1β的表达。
Western blot 分析显示，CD 回肠和 UC 直肠活检组织中 CB2 的表达显著增加，UC 直肠中 TLR4 的表达增加。我们还观察到 UC 直肠中 IL-6 的表达显著上调。免疫荧光分析证实了 Western blot 数据，还显示了 T 淋巴细胞浸润与 CD 回肠和 UC 直肠中 CB2 表达的共定位。
我们的结果表明，儿科 IBD 中 CB2 的表达上调，这可能对药物发现具有重要意义。
大麻素受体 2 型（CB2）参与儿科炎症性肠病（IBD）的炎症和免疫反应调节。CB2 受体在儿科 IBD 患者的炎症肠道中表达更高。CB2 可作为一种潜在的治疗靶点，用于减轻儿童期与 IBD 相关的炎症状态。
