Shi Guo-Ru, Liang Rui-Lan, Yu Shi-Shan
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(11):2989-2993. doi: 10.19540/j.cnki.cjcmm.20220412.201.
The present study investigated the chemical constituents from the dry seeds of Hydnocarpus anthelminthica. The compounds were isolated and purified from the dry seeds of H. anthelminthica by various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC. Their structures were identified by spectroscopic analysis. The in vitro cytotoxic activities were determined by MTT assay. Ten compounds were isolated and identified as 2-(4-hydroxy-3,5-dimethoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propanol(1), threo-1,2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1,3-diol(2), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1,3-diol(3), 2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propanol(4), 3-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)-propan-1-one(5), chrysoeriol(6), evofolin B(7), apigenin-3'-methoxy-7-O-rutinoside(8), luteolin(9), and vitexin(10). Compound 1 is a new compound. Compounds 4 and 5 were isolated from this genus for the first time. All compounds showed no significant cytotoxic activity.
本研究对大风子干燥种子的化学成分进行了研究。通过各种色谱技术，包括硅胶柱色谱、Sephadex LH - 20柱色谱和反相高效液相色谱，从大风子干燥种子中分离并纯化了这些化合物。通过光谱分析确定了它们的结构。采用MTT法测定了其体外细胞毒性活性。分离并鉴定出10种化合物，分别为2-(4-羟基-3,5-二甲氧基苯基)-3-(2-羟基-5-甲氧基苯基)-3-氧代-1-丙醇(1)、苏式-1,2-双-(4-羟基-3-甲氧基苯基)-丙烷-1,3-二醇(2)、赤式-1,2-双-(4-羟基-3-甲氧基苯基)-丙烷-1,3-二醇(3)、2-(4-羟基-3-甲氧基苯基)-3-(2-羟基-5-甲氧基苯基)-3-氧代-1-丙醇(4)、3-羟基-2-(4-羟基-3-甲氧基苯基)-1-(4-羟基苯基)-丙-1-酮(5)、芹菜素(6)、异佛林B(7)、芹菜素-3'-甲氧基-7-O-芸香糖苷(8)、木犀草素(9)和牡荆素(10)。化合物1是一种新化合物。化合物4和5首次从该属中分离得到。所有化合物均未表现出显著的细胞毒性活性。
