Introgression of the Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers.

(2 = 28, A A GG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat (2 = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat- introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the A and G subgenomes of . These introgressions contained 89 and 74 unique segments from the A and G subgenomes, respectively. These overlapping segments covered 98.9% of the genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all chromosomes varying sized segments except for chromosomes 3A , 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of A majority of these KASP markers were also found to be subgenome specific with 182 detecting A subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the A segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour hybridisation analysis. Together these homozygous wheat- ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.