Yadav Rakesh Kumar, Bharti Divya, Tikku Aseem Prakash, Verma Promila, Shakya Vijay Kumar, Pandey Pragya
Department of Conservative Dentistry and Endodontics, King George's Medical University, Lucknow, Uttar Pradesh, India.
J Conserv Dent. 2022 Jan-Feb;25(1):26-31. doi: 10.4103/jcd.jcd_426_21. Epub 2022 May 2.
The aim of the study is to evaluate and compare the remineralization efficacy of remineralizing agents, i.e., fluoride-free toothpaste, fluoride toothpaste, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), CPP-amorphous calcium fluoride phosphate (CPP ACFP), and silver diamine fluoride (SDF) paste on dentine over time.
Seventy-five extracted human permanent molars were selected. The buccal section of these samples was shaped into a slab. Artificial caries was induced by dipping the samples into the demineralizing solution (pH 4.5) for 21 days. They were then divided into five groups ( = 15). Each experimental group underwent one of the following treatments: Group 1 - Fluoride-free toothpaste (negative control), Group 2 - Fluoride toothpaste (positive control), Group 3 - CPP-ACP, Group 4 - CPP-Amorphous Calcium fluoride Phosphate and Group 5 - SDF. Postremineralizing treatment, Scanning electron microscope, Energy dispersive X-ray and Quantitative light induced fluorescence imaging were carried out to analyze the remineralizing efficacy. The data obtained was then subjected to statistical analysis using ANOVA and Paired -tests.
It was seen that SDF showed highest remineralization efficacy followed by CPP-amorphous calcium fluoride phosphate, CPP-ACP, fluoride toothpaste and fluoride-free toothpaste. The difference in mean value among the groups was statistically significant ( < 0.001).
SDF showed the highest remineralizing potential in scanning electron microscopy and energy dispersive X-ray, followed by CPP-ACFP, CPP-ACP, Fluoride toothpaste, and Fluoride-free toothpaste. Quantitative light fluorescence analysis showed more fluorescence changes in the CPP-ACFP followed by CPP-ACP, Fluoride toothpaste, and Fluoride-free toothpaste.
本研究旨在评估并比较不同再矿化剂，即无氟牙膏、含氟牙膏、酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）、CPP-无定形氟化磷酸钙（CPP ACFP）和氟化银胺（SDF）糊剂对牙本质随时间的再矿化效果。
选取75颗拔除的人类恒牙磨牙。将这些样本的颊面制成平板状。通过将样本浸入脱矿溶液（pH 4.5）中21天诱导人工龋。然后将它们分为五组（每组n = 15）。每个实验组接受以下一种处理：第1组 - 无氟牙膏（阴性对照），第2组 - 含氟牙膏（阳性对照），第3组 - CPP-ACP，第4组 - CPP-无定形氟化磷酸钙，第5组 - SDF。再矿化处理后，进行扫描电子显微镜、能量色散X射线和定量光诱导荧光成像分析再矿化效果。然后使用方差分析和配对t检验对获得的数据进行统计分析。
结果显示，SDF的再矿化效果最高，其次是CPP-无定形氟化磷酸钙、CPP-ACP、含氟牙膏和无氟牙膏。各组平均值之间的差异具有统计学意义（P < 0.001）。
在扫描电子显微镜和能量色散X射线中，SDF显示出最高的再矿化潜力，其次是CPP-ACFP、CPP-ACP、含氟牙膏和无氟牙膏。定量光荧光分析显示，CPP-ACFP的荧光变化更多，其次是CPP-ACP、含氟牙膏和无氟牙膏。
