Gaikwad Swapnil, Birla Sonal, Ingle Avinash P, Gade Aniket, Ingle Pramod, Golińska Patrycja, Rai Mahendra
Department of Biotechnology, SGB Amravati University, Amravati, India.
Microbial Diversity Research Center, Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, India.
Front Microbiol. 2022 Jun 2;13:881404. doi: 10.3389/fmicb.2022.881404. eCollection 2022.
Wound healing is a complex phenomenon particularly owing to the rise in antimicrobial resistance. This has attracted the attention of the scientific community to search for new alternative solutions. Among these, silver being antimicrobial has been used since ancient times. Considering this fact, the main goal of our study was to evaluate the wound-healing ability of mycofabricated silver nanoparticles (AgNPs). We have focused on the formulation of silver nanogel for the management of wounds in albino Wistar rats. Mycosynthesized AgNPs from were used for the development of novel wound-healing antimicrobial silver nanogel with different concentrations of AgNPs, i.e., 0.1, 0.5, and 1 mg g. The formulated silver nanogel demonstrated excellent wound-healing activity in the incision, excision, and burn wound-healing model. In the incision wound-healing model, silver nanogel at a concentration of 0.5 mg g exhibited superior wound-healing effect, whereas in the case of excision and burn wound-healing model, silver nanogel at the concentrations of 0.1 and 1 mg g showed enhanced wound-healing effect, respectively. Moreover, silver nanogel competently arrests the bacterial growth on the wound surface and offers an improved local environment for scald wound healing. Histological studies of healed tissues and organs of the rat stated that AgNPs at less concentration (1 mg g) do not show any toxic or adverse effect on the body and promote wound healing of animal tissue. Based on these studies, we concluded that the silver nanogel prepared from mycosynthesized AgNPs can be used as a promising antimicrobial wound dressing.
伤口愈合是一种复杂的现象，尤其是由于抗菌耐药性的增加。这引起了科学界的关注，以寻找新的替代解决方案。其中，具有抗菌作用的银自古以来就被使用。考虑到这一事实，我们研究的主要目标是评估真菌合成的银纳米颗粒（AgNPs）的伤口愈合能力。我们专注于制备用于白化Wistar大鼠伤口处理的银纳米凝胶。从[具体来源未给出]真菌合成的AgNPs被用于开发具有不同AgNPs浓度（即0.1、0.5和1mg/g）的新型伤口愈合抗菌银纳米凝胶。所制备的银纳米凝胶在切口、切除和烧伤伤口愈合模型中均表现出优异的伤口愈合活性。在切口伤口愈合模型中，浓度为0.5mg/g的银纳米凝胶表现出卓越的伤口愈合效果，而在切除和烧伤伤口愈合模型中，浓度为0.1和1mg/g的银纳米凝胶分别表现出增强的伤口愈合效果。此外，银纳米凝胶能够有效抑制伤口表面的细菌生长，并为烫伤伤口愈合提供改善的局部环境。对大鼠愈合组织和器官的组织学研究表明，较低浓度（1mg/g）的AgNPs对身体没有显示出任何毒性或不良影响，并促进动物组织的伤口愈合。基于这些研究，我们得出结论，由真菌合成的AgNPs制备的银纳米凝胶可作为一种有前景的抗菌伤口敷料。
