Max Planck Institute for Multidisciplinary Sciences, NMR Signal Enhancement Group, Am Fassberg 11, 37077, Göttingen, Germany.
Center for Biostructural Imaging of Neurodegeneration of UMG, NMR Signal Enhancement Group, Von-Siebold-Straße 3 A, 37075, Göttingen, Germany.
Angew Chem Int Ed Engl. 2022 Aug 22;61(34):e202206298. doi: 10.1002/anie.202206298. Epub 2022 Jul 14.
Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real-time investigations of dynamic processes in vitro and in vivo. Here, we propose a field-independent para-hydrogen-based pulsed method to produce rapidly hyperpolarized C-labeled substrates. We demonstrate the method by polarizing the carboxylic carbon of the pyruvate moiety in a purposely designed precursor to 24 % at ≈22 mT. Following a fast purification procedure, we measure 8 % polarization on free [1- C]pyruvate in clean water solutions at physiological conditions at 7 T. The enhanced signals allow real-time monitoring of the pyruvate-lactate conversion in cancer cells, demonstrating the potential of the method for biomedical applications in combination with existing or developing magnetic resonance technologies.
磁共振中的极化方法将信号增强了几个数量级，为体外和体内动态过程的实时研究开辟了新的窗口。在这里，我们提出了一种场独立的基于仲氢的脉冲方法，用于产生快速极化的 C 标记底物。我们通过将设计好的前体中丙酮酸部分的羧酸碳极化到 ≈22 mT 时的 24 %来证明该方法。经过快速纯化程序，我们在 7 T 下的生理条件下，在清洁水溶剂中测量到游离[1- C]丙酮酸的 8 %极化。增强的信号允许实时监测癌细胞中的丙酮酸-乳酸转化，表明该方法与现有或开发中的磁共振技术结合，在生物医学应用方面具有潜力。
