Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden.
Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden.
Bioresour Technol. 2022 Sep;359:127494. doi: 10.1016/j.biortech.2022.127494. Epub 2022 Jun 17.
Biorefineries enable the circular, sustainable, and economic use of waste resources if value-added products can be recovered from all the generated fractions at a large-scale. In the present studies the comparison and assessment for the production of value-added compounds (e.g., proteins, lutein, and lipids) by the microalga Chlorella sorokiniana grown under photoautotrophic or heterotrophic conditions was performed. Photoautotrophic cultivation generated little biomass and lipids, but abundant proteins (416.66 mg/g) and lutein (6.40 mg/g). Heterotrophic conditions using spruce hydrolysate as a carbon source favored biomass (8.71 g/L at C/N 20 and 8.28 g/L at C/N 60) and lipid synthesis (2.79 g/L at C/N 20 and 3.61 g/L at C/N 60) after 72 h of cultivation. Therefore, heterotrophic cultivation of microalgae using spruce hydrolysate instead of glucose offers a suitable biorefinery concept at large-scale for biodiesel-grade lipids production, whereas photoautotrophic bioreactors are recommended for sustainable protein and lutein biosynthesis.
如果能够从所有产生的部分中以大规模回收增值产品，则生物精炼厂可以实现废物资源的循环、可持续和经济利用。在本研究中，对在光自养或异养条件下生长的小球藻（Chlorella sorokiniana）生产增值化合物（例如蛋白质、叶黄素和脂质）进行了比较和评估。光自养培养产生的生物量和脂质很少，但富含蛋白质（416.66 mg/g）和叶黄素（6.40 mg/g）。使用云杉水解物作为碳源的异养条件有利于生物质（C/N 20 时为 8.71 g/L，C/N 60 时为 8.28 g/L）和脂质合成（C/N 20 时为 2.79 g/L，C/N 60 时为 3.61 g/L），培养 72 小时后。因此，使用云杉水解物代替葡萄糖进行异养培养微藻为大规模生产生物柴油级脂质提供了合适的生物精炼概念，而光自养生物反应器则推荐用于可持续的蛋白质和叶黄素生物合成。
