LINC01088 通过 ceRNA 机制调节 miR-95/LATS2 通路抑制胃癌细胞的生长、侵袭和迁移。
LINC01088 regulates the miR-95/LATS2 pathway through the ceRNA mechanism to inhibit the growth, invasion and migration of gastric cancer cells.
机构信息
Quality control office, 609245The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China.
Third department of Surgery, 609245The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China.
出版信息
Int J Immunopathol Pharmacol. 2022 Jan-Dec;36:3946320221108271. doi: 10.1177/03946320221108271.
In gastric cancer, a malignant condition with a dismal prognosis, long non-coding RNAs (LncRNAs) play a significant regulatory role. They often compete with microRNAs through the ceRNA mechanism to affect the expression of target mRNA. However, the specific clinical value and mechanism of action of LncRNA in gastric cancer are still unclear. This study detected the expression and clinical value of LINC01088 in gastric cancer tissues. Furthermore, the biological functions of LINC01088 and the regulation mechanism of the miR-95/LATS2 pathway were explored. LINC01088 and LATS2 mRNA expression decreased, and miR-95 increased in gastric cancer tissues. LINC01088 has an excellent positive correlation with LATS2 mRNA, which may be a ceRNA pair; LINC01088 has binding sites with miR-95. Gene interference tests on gastric cancer cell lines revealed that LINC01088 could prevent gastric cancer cells from proliferating, invading, and migrating. The function of LINC01088 is achieved by regulating the miR-95/LATS2 pathway through the ceRNA mechanism. The results of this study show that LINC01088 expression is significantly reduced in gastric cancer tissues and cell lines. LINC01088 inhibits gastric cancer cells' proliferation, invasion, and migration by regulating the miR-95/LATS2 pathway via the ceRNA mechanism.
在胃癌这种预后不良的恶性疾病中，长链非编码 RNA（lncRNA）发挥着重要的调控作用。它们常通过 ceRNA 机制与 microRNA 竞争，从而影响靶 mRNA 的表达。然而，lncRNA 在胃癌中的具体临床价值和作用机制仍不明确。本研究检测了 linc01088 在胃癌组织中的表达及其临床价值，进一步探讨了 linc01088 对 miR-95/LATS2 通路的调控机制及其生物学功能。结果显示，胃癌组织中 linc01088 和 LATS2mRNA 表达下调，miR-95 表达上调。LINC01088 与 LATS2mRNA 呈极好的正相关，可能是一对 ceRNA；LINC01088 与 miR-95 存在结合位点。对胃癌细胞系进行基因干扰实验发现，linc01088 可阻止胃癌细胞增殖、侵袭和迁移。linc01088 通过 ceRNA 机制调控 miR-95/LATS2 通路发挥功能。本研究结果表明，linc01088 在胃癌组织和细胞系中表达显著下调。linc01088 通过 ceRNA 机制调控 miR-95/LATS2 通路，抑制胃癌细胞的增殖、侵袭和迁移。
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