Department of Dermatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
J Cosmet Dermatol. 2023 Feb;22(2):505-511. doi: 10.1111/jocd.15175. Epub 2022 Jul 18.
Ultraviolet B (UVB) affects diverse pathways in skin cells, resulting in skin photoaging. Skin fibroblasts internalize and degrade elastin and collagen, playing prominent roles in photoaging. Green light is used in many fields of dermatology, but few studies have examined its role in photoaging. The present work aimed to assess low-energy green light for its effects in a previously proposed cell model of photoaging and to explore the possible anti-photoaging mechanism.
The stress-induced premature senescence (SIPS) model was constructed via repeated treatment of MDFs with UVB. Senescence-like phenotypes were compared among normal, low-energy green light pretreatment and UVB groups, for example, cell morphological properties, senescence-associated β-galactosidase (SA-β-gal) amounts, extracellular matrix (ECM) biosynthesis and degradation, and autophagy.
In comparison with the UVB group, the green light pretreatment group showed significantly decreased number of senescent mast cells and markedly declined signal intensity and amounts of SA-β-gal-positive cells. Furthermore, green light pretreatment directly affected ECM by increasing type I and type III collagen production and decreasing MMP-1 amounts. Moreover, changes in autophagy levels induced by green light pretreatment provided a potential mechanism underlying its anti-aging property.
Low-energy green light pretreatment improves senescence-like phenotypes in vitro, indicating a possible application for anti-aging in clinic after future research has uncovered the potential mechanism.
中波紫外线（UVB）会影响皮肤细胞中的多种途径，导致皮肤光老化。皮肤成纤维细胞内化和降解弹性蛋白和胶原蛋白，在光老化中发挥重要作用。绿光在皮肤科的许多领域都有应用，但很少有研究探讨其在光老化中的作用。本研究旨在评估低能量绿光对先前提出的光老化细胞模型的作用，并探讨其可能的抗光老化机制。
通过重复用 UVB 处理 MDF 来构建应激诱导的早衰（SIPS）模型。比较正常组、低能量绿光预处理组和 UVB 组之间的衰老样表型，例如细胞形态特征、衰老相关β-半乳糖苷酶（SA-β-gal）含量、细胞外基质（ECM）生物合成和降解以及自噬。
与 UVB 组相比，绿光预处理组衰老 mast 细胞数量明显减少，SA-β-gal 阳性细胞的信号强度和数量明显下降。此外，绿光预处理直接影响 ECM，增加 I 型和 III 型胶原蛋白的产生，减少 MMP-1 的含量。此外，绿光预处理引起的自噬水平的变化为其抗老化特性提供了一个潜在的机制。
低能量绿光预处理可改善体外衰老样表型，提示在未来的研究揭示潜在机制后，绿光预处理可能具有临床应用于抗衰老的潜力。
