Max-Planck-Institute for Polymer Research, Ackermannweg 10, Mainz 55122, Germany; Department of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraβe 1, Mainz 55131, Germany.
Max-Planck-Institute for Polymer Research, Ackermannweg 10, Mainz 55122, Germany.
Acta Biomater. 2022 Aug;148:271-278. doi: 10.1016/j.actbio.2022.06.028. Epub 2022 Jun 19.
The composition of the protein corona varies depending on several parameters and influences the cellular fate of the nanocarriers. Here, we investigated the influence of three key parameters (surface charge, temperature, and plasma concentration) on the formation and composition of the protein corona of polystyrene nanoparticles and ultimately on the cellular uptake of pre-coated nanoparticles. At a fixed temperature and concentration, the surface charge, and surfactant influence its composition. We observed that the composition of the corona formed at low temperatures (4°C) is different from that formed at physiological temperatures (37°C). At low plasma concentrations (up to 25%), the corona consists of more diverse proteins than at higher concentrations. Finally, we concluded that regardless of the nanoparticle formulation, the degree of uptake by model cancer and endothelial cells of the nanoparticles decreased when pre-coated at increasing temperature or plasma concentration. STATEMENT OF SIGNIFICANCE: Drug delivery through nanocarriers is an increasingly important concept in research and medicine. One problem in the application of nanocarriers in medicine is the protein corona that forms around the nanocarriers when they get in contact with protein-containing fluids. So far, several factors have been identified that influence the composition of the protein corona and thus the biological identity of the particles. However, lacking comparability remains between the studies because different concentrations or temperatures of the protein solutions are used. In this study we demonstrate how the incubation temperature or the concentration of plasma influences the protein corona and thus the cellular uptake of polystyrene nanoparticles.
蛋白质冠的组成取决于几个参数，并影响纳米载体的细胞命运。在这里，我们研究了三个关键参数（表面电荷、温度和血浆浓度）对聚苯乙烯纳米颗粒蛋白质冠的形成和组成的影响，以及对预涂覆纳米颗粒的细胞摄取的影响。在固定温度和浓度下，表面电荷和表面活性剂会影响其组成。我们观察到，在低温（4°C）下形成的冠层的组成与在生理温度（37°C）下形成的冠层的组成不同。在低血浆浓度（高达 25%）下，形成的冠层中的蛋白质比在更高浓度下更为多样化。最后，我们得出结论，无论纳米颗粒制剂如何，当在升高的温度或血浆浓度下预涂覆时，模型癌细胞和内皮细胞对纳米颗粒的摄取程度都会降低。
通过纳米载体进行药物输送是研究和医学中日益重要的概念。在医学中应用纳米载体的一个问题是，当纳米载体与含有蛋白质的液体接触时，会在其周围形成蛋白质冠。到目前为止，已经确定了几个因素会影响蛋白质冠的组成，从而影响颗粒的生物学特性。然而，由于使用了不同浓度或温度的蛋白质溶液，研究之间仍然缺乏可比性。在这项研究中，我们展示了孵育温度或血浆浓度如何影响蛋白质冠，从而影响聚苯乙烯纳米颗粒的细胞摄取。
