Department of Clinical Laboratory, The First People's Hospital of Linhai, Taizhou, Zhejiang, China.
Department of Clinical Laboratory, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
J Glob Antimicrob Resist. 2022 Sep;30:148-151. doi: 10.1016/j.jgar.2022.06.014. Epub 2022 Jun 19.
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a prevalent pathogen contributing to hospital infections. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and core-genome MLST (cgMLST) are frequently used methods to illuminate the nosocomial transmission of CRAB. In this study, we compared the discriminatory power of the three typing methods.
Antimicrobial susceptibility tests were performed by the broth microdilution and Vitek2 methods. PFGE, MLST and cgMLST were conducted to determine the clonality and phylogenetic relationship of the strains. Whole-genome sequence data were acquired by an Illumina HiSeq 2000, and cgMLST was analysed by the Ridom SeqSphere+ v.7.2.3 software.
A total of 149 carbapenem-resistant A. baumannii isolates had 15 different PFGE profiles (A-O type), and 73 of the isolates had related subtypes (A1 and A2), accounting for the majority of type A isolates. The maximum-likelihood phylogenetic analysis based on the cgMLST genes grouped the same PFGE clonal pattern A into nine different clusters. ST_Pasteur grouped all the strains into ST2, whereas ST_Oxford grouped the PFGE clonal pattern A isolates into six STs. In addition, the gdhB allele in the ST_Oxford scheme had two copies in five strains, which complicated the ST_Oxford typing.
cgMLST was more discriminant than PFGE and MLST. CgMLST is the most suitable and comprehensive method for genotyping A. baumannii in surveillance and epidemiological research.
耐碳青霉烯鲍曼不动杆菌（CRAB）是一种流行的病原体，导致医院感染。脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）和核心基因组 MLST（cgMLST）常用于阐明 CRAB 的医院内传播。在这项研究中，我们比较了这三种分型方法的分辨能力。
采用肉汤微量稀释法和 Vitek2 方法进行药敏试验。PFGE、MLST 和 cgMLST 用于确定菌株的克隆性和系统发育关系。通过 Illumina HiSeq 2000 获得全基因组序列数据，并通过 Ridom SeqSphere+ v.7.2.3 软件分析 cgMLST。
共分离到 149 株耐碳青霉烯鲍曼不动杆菌，有 15 种不同的 PFGE 图谱（A-O 型），其中 73 株为相关亚型（A1 和 A2），占 A 型菌株的大多数。基于 cgMLST 基因的最大似然系统发育分析将相同 PFGE 克隆模式 A 分为九个不同的聚类。ST_Pasteur 将所有菌株分为 ST2，而 ST_Oxford 将 PFGE 克隆模式 A 菌株分为六个 ST。此外，ST_Oxford 方案中的 gdhB 等位基因在五个菌株中有两个拷贝，这使得 ST_Oxford 分型复杂化。
cgMLST 比 PFGE 和 MLST 更具分辨力。cgMLST 是监测和流行病学研究中用于鲍曼不动杆菌基因分型的最适合和全面的方法。
