Department of Animal Medicine, College of Life Sciences and Food Engineering, Hebei University of Engineering, No. 19 Taiji Road, Handan, 056038, China.
Arch Microbiol. 2022 Jun 22;204(7):413. doi: 10.1007/s00203-022-02838-5.
A novel lytic Enterococcus faecalis phage, EFC1, was isolated from the sewage of a farm in Handan, China, and its genome was analyzed and described. The phage could infect 87.5% of the chicken-derived Enterococcus faecalis preserved in our laboratory. The genome of phage EFC1 consists of a circular double-stranded DNA with a length of 56,099 bp and a G + C content of 39.96%, containing 89 predicted protein-coding genes as well as 2 tRNAs, which are involved in phage intron, structure, transcription, packaging, DNA replication, modification, cell lysis, and other functions, indicating the genetic and functional characteristics of this phage. Genome comparison analysis revealed that phage EFC1 can be regarded as new genus Saphexavirus phage in the Siphoviridae family.
一株来自中国邯郸某农场污水的溶血性粪肠球菌噬菌体 EFC1 被分离并对其基因组进行了分析和描述。该噬菌体可以感染本实验室保存的 87.5%的鸡源粪肠球菌。噬菌体 EFC1 的基因组是一个长度为 56099bp 的环状双链 DNA，G+C 含量为 39.96%，包含 89 个预测的蛋白编码基因和 2 个 tRNA，这些基因参与噬菌体内含子、结构、转录、包装、DNA 复制、修饰、细胞裂解等功能，表明了该噬菌体的遗传和功能特征。基因组比较分析表明，噬菌体 EFC1 可以被认为是 Siphoviridae 科 Saphexavirus 属的新噬菌体。
