Meijer A E, Israël D E
Histochemistry. 1978 Aug 15;57(1):23-31. doi: 10.1007/BF00507353.
The reliability of enzyme histochemical semipermeable membrane techniques for the demonstration of acid hydrolases was investigated with a combined histochemical and biochemical study. In part 1 the histochemical findings were presented. In this communication the biochemical findings are reported and compared with the histochemical findings. In m. soleus, m. plantaris, m. gastrocnemius and diaphragm of vitamin E deficient rabbits the activity of the lysosomal acid hydrolases, cathepsin D, acid maltase, acid phosphatase and beta-glucuronidase is significantly increased. This increase in activity of the investigated acid hydrolases was equal for muscles with an aerobic or an anaerobic metabolism. By means of statistical calculations the activity of the enzymes demonstrated with histochemical techniques was compared with the enzyme activity determined with biochemical techniques. From the results of this investigation it can be concluded that the histochemical semipermeable membrane techniques for the demonstration of activity of acid hydrolases are very reliable. Considering the fact that these techniques are also tissue-saving, they are therefore extremely suitable for the study of catabolic wasting processes in skeletal muscle tissues of patients with inherited or acquired muscular diseases.
通过组织化学和生物化学相结合的研究，对用于显示酸性水解酶的酶组织化学半透膜技术的可靠性进行了调查。在第1部分中展示了组织化学研究结果。在本报告中，将报告生物化学研究结果并与组织化学研究结果进行比较。在维生素E缺乏的兔子的比目鱼肌、跖肌、腓肠肌和膈肌中，溶酶体酸性水解酶、组织蛋白酶D、酸性麦芽糖酶、酸性磷酸酶和β-葡萄糖醛酸酶的活性显著增加。所研究的酸性水解酶活性的增加在有氧或无氧代谢的肌肉中是相同的。通过统计计算，将组织化学技术显示的酶活性与生物化学技术测定的酶活性进行了比较。从这项研究结果可以得出结论，用于显示酸性水解酶活性的组织化学半透膜技术非常可靠。鉴于这些技术还节省组织，因此它们极其适合用于研究患有遗传性或获得性肌肉疾病患者骨骼肌组织中的分解代谢消耗过程。
