Lojda Z, Gutmann E
Histochemistry. 1976 Nov 12;49(4):337-42. doi: 10.1007/BF00496137.
The distribution of acid phosphatase, beta-N-acetylglucosaminidase, beta-glucuronidase, and acid beta-galactosidase was studied in mm. extensor digitorum longus, soleus, and diaphragm of rats. Using the technic of semipermeable membranes activities of these enzymes were demonstrated beside cells of the interstitial tissue in muscle fibers themselves as well. Acid phosphatase displayed the highest activity which appeared in many small dots dispersed in the fiber. The activity of acid phosphatase was about 1.2 X higher in the m. soleus than in the m. extensor digitorum longus. In the latter muscle a somewhat higher activity was often found in muscle fibers displaying a higher staining for NADH tetrazolium reductase. The activity of beta-N-acetylglucosaminidase was slightly lower, that of beta-glucuronidase very weak but still discernible. The activity of acid beta-galactosidase was not ascertained in the majority of fibers. The ratio of activities measured in an area of the same size in cells of the interstitial tissue and in muscle fibers amounted in average to 2.6:1 in the case of acid phosphatase, 2.5:1 in the case of beta-N-acetylglucosaminidase, 5.7:1 in the case of beta-glucuronidase, and 44.3:1 in the case of acid beta-galactosidase. The importance of the histochemical technic in studies concerned with acid hydrolases in striated muscle fibers in normal and pathological conditions is pointed out.
研究了大鼠趾长伸肌、比目鱼肌和膈肌中酸性磷酸酶、β-N-乙酰氨基葡萄糖苷酶、β-葡萄糖醛酸酶和酸性β-半乳糖苷酶的分布。采用半透膜技术，在肌纤维自身的间质组织细胞以及肌纤维中均证实了这些酶的活性。酸性磷酸酶活性最高，呈许多散在纤维中的小点状出现。比目鱼肌中酸性磷酸酶的活性比趾长伸肌高约1.2倍。在后者的肌肉中，经常发现NADH四唑还原酶染色较高的肌纤维中酸性磷酸酶活性略高。β-N-乙酰氨基葡萄糖苷酶的活性略低，β-葡萄糖醛酸酶的活性非常弱但仍可辨别。大多数纤维中未确定酸性β-半乳糖苷酶的活性。间质组织细胞和肌纤维中相同大小区域内测得的酶活性之比，酸性磷酸酶平均为2.6:1，β-N-乙酰氨基葡萄糖苷酶为2.5:1，β-葡萄糖醛酸酶为5.7:1，酸性β-半乳糖苷酶为44.3:1。指出了组织化学技术在正常和病理条件下与横纹肌纤维中酸性水解酶相关研究中的重要性。
