Department of Chemistry, Punjab Agricultural University, Ludhiana 141004, India.
Department of Food and Nutrition, Punjab Agricultural University, Ludhiana 141004, India.
Biosensors (Basel). 2022 Jun 17;12(6):428. doi: 10.3390/bios12060428.
Polyphenol oxidase (PPO) mimics have advantage of detection and remediation of polyphenols. This work demonstrates rapid and sensitive colorimetric detection of phenolic compounds using nanocomposite of magnesium ferrite (MgFeO) and manganese oxide (MnO) nanoparticles as PPO mimic. The catalytic properties of MgFeO and MnO displayed synergistic effect in the nanocomposite. The synthesized nanocomposite and nanoparticles were fully characterized using various analytical techniques. The ratio of MgFeO and MnO in the nanocomposite was optimized. Catechol and resorcinol were taken as model polyphenols. The best PPO-activity was shown by MgFeO@MnO nanocomposite with of / ratio 1:2. The results correlated with its higher surface area. Reaction parameters viz. pH, temperature, contact time, substrate concentration, and nanoparticles dose were studied. The synthesized MgFeO@MnO nanocomposite was used for the detection of catechol in the linear range of 0.1-0.8 mM with the detection limit of 0.20 mM, and resorcinol in the range of 0.01-0.08 mM with the detection limit of 0.03 mM. The estimated total phenolic content of green and black tea correlated well with the conventional method. These results authenticate promising future potential of MgFeO@MnO nanocomposite as PPO-mimic.
多酚氧化酶（PPO）模拟物在多酚的检测和修复方面具有优势。本工作利用镁铁氧体（MgFeO）和氧化锰（MnO）纳米粒子的纳米复合材料作为 PPO 模拟物，快速灵敏地检测酚类化合物。MgFeO 和 MnO 的催化性能在纳米复合材料中显示出协同效应。使用各种分析技术对合成的纳米复合材料和纳米粒子进行了全面的表征。优化了纳米复合材料中 MgFeO 和 MnO 的比例。儿茶酚和间苯二酚被用作模型多酚。/比例为 1:2 的 MgFeO@MnO 纳米复合材料表现出最佳的 PPO 活性。结果与其更高的表面积相关。研究了反应参数，如 pH 值、温度、接触时间、底物浓度和纳米粒子剂量。合成的 MgFeO@MnO 纳米复合材料用于检测儿茶酚，线性范围为 0.1-0.8 mM，检测限为 0.20 mM，间苯二酚的检测范围为 0.01-0.08 mM，检测限为 0.03 mM。绿茶和红茶的总酚含量与传统方法相关性良好。这些结果证实了 MgFeO@MnO 纳米复合材料作为 PPO 模拟物的有前途的未来潜力。
