Ling Yi, Chen Liuyuchen, Huang Mingjun, Zhou Cheng, Yang Liming, Niu Hejingying, Su Li, Yang Yuejiao, Pirraco Rogério P, Reis Rui L, Chen Jie
Department of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, China.
School of Life Sciences, Shanghai University, Shangda Road 99, Shanghai 200444, China.
Gels. 2022 May 31;8(6):345. doi: 10.3390/gels8060345.
In an attempt to find a potential application of cell culture harvesting, a novel method for the preparation of an upper critical solution temperature (UCST) thermosensitive hydrogel was studied. An electron accelerator was used as the electron beam (EB) radiation source, and acrylamide (AAm) was first grafted onto the pre-irradiated polypropylene (PP) sheet. Then, the grafting layer of poly (acrylamide-co-acrylonitrile) (P (AAm-co-AN)) was obtained by the partial dehydration of the acylamino group into the cyano group in the solution mixture of sulfoxide chloride (SOCl) and dimethyl formamide (DMF). The effects of the absorbed dose, AAm concentration, reaction time, and temperature on the degree of grafting were studied, respectively. The effect of the SOCl concentration on the conversion degree of the cyano group from the acylamino group was studied, followed by the temperature of the UCST. The UCST properties of the grafted samples with P (AAm-co-AN) were studied by quartz crystal microbalance (QCM) and atomic force microscope (AFM), respectively. The cytotoxicities of the hydrogels against cells were verified by CCK-8 studies.
为了探索细胞培养收获的潜在应用，研究了一种制备上临界溶液温度（UCST）热敏水凝胶的新方法。使用电子加速器作为电子束（EB）辐射源，首先将丙烯酰胺（AAm）接枝到预辐照的聚丙烯（PP）片材上。然后，通过在亚硫酰氯（SOCl）和二甲基甲酰胺（DMF）的溶液混合物中将酰氨基部分脱水成氰基，得到聚（丙烯酰胺 - 丙烯腈）（P（AAm - co - AN））接枝层。分别研究了吸收剂量、AAm浓度、反应时间和温度对接枝度的影响。研究了SOCl浓度对酰氨基转化为氰基转化率的影响，随后研究了UCST温度。分别通过石英晶体微天平（QCM）和原子力显微镜（AFM）研究了接枝有P（AAm - co - AN）的样品的UCST性质。通过CCK - 8研究验证了水凝胶对细胞的细胞毒性。
