Sangkert Supaporn, Juncheed Kantida, Meesane Jirut
Institute of Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand.
J Funct Biomater. 2022 Jun 14;13(2):80. doi: 10.3390/jfb13020080.
Osteoconductive silk fibroin (SF) binders were fabricated for the bone repair of an alveolar cleft defect. Binders were prefigureared by mixing different ratios of a mixture of random coils and SF aggregation with SF fibrils: 100:0 (SFB100), 75:25 (SFB75), 50:50 (SFB50), 25:75 (SFB25), and 0:100 (SFB0). The gelation, molecular organization, structures, topography, and morphology of the binders were characterized and observed. Their physical, mechanical, and biological properties were tested. The SF binders showed gelation via self-assembly of SF aggregation and fibrillation. SFB75, SFB50, and SFB25 had molecular formation via the amide groups and showed more structural stability than SFB100. The morphology of SFB0 demonstrated the largest pore size. SFB0 showed a lowest hydrophilicity. SFB100 showed the highest SF release. SFB25 had the highest maximum load. SFB50 exhibited the lowest elongation at break. Binders with SF fibrils showed more cell viability and higher cell proliferation, ALP activity, calcium deposition, and protein synthesis than without SF fibrils. Finally, the results were deduced: SFB25 demonstrated suitable performance that is promising for the bone repair of an alveolar cleft defect.
制备了骨传导性丝素蛋白（SF）粘合剂用于牙槽嵴裂缺损的骨修复。通过将不同比例的无规卷曲和SF聚集体与SF原纤维的混合物混合来制备粘合剂：100:0（SFB100）、75:25（SFB75）、50:50（SFB50）、25:75（SFB25）和0:100（SFB0）。对粘合剂的凝胶化、分子组织、结构、形貌和形态进行了表征和观察。测试了它们的物理、力学和生物学性能。SF粘合剂通过SF聚集体的自组装和原纤化表现出凝胶化。SFB75、SFB50和SFB25通过酰胺基团形成分子，并且比SFB100表现出更高的结构稳定性。SFB0的形貌显示出最大的孔径。SFB0表现出最低的亲水性。SFB100表现出最高的SF释放量。SFB25具有最高的最大负荷。SFB50在断裂时表现出最低的伸长率。与不含SF原纤维的粘合剂相比，含SF原纤维的粘合剂表现出更高的细胞活力、更高的细胞增殖、碱性磷酸酶活性、钙沉积和蛋白质合成。最后，得出结果：SFB25表现出适合牙槽嵴裂缺损骨修复的性能，具有良好的前景。
