Ogrinc Nina, Schneider Serge, Bourmaud Adèle, Gengler Nicolas, Salzet Michel, Fournier Isabelle
Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Inserm U1192, Université de Lille, F-59000 Lille, France.
Service de Toxicologie Analytique-Chimie Pharmaceutique, Laboratoire National de Santé (LNS), Dudelange, L-3555 Luxembourg, Luxembourg.
Metabolites. 2022 May 26;12(6):480. doi: 10.3390/metabo12060480.
In recent years, cannabis and hemp-based products have become increasingly popular for recreational use, edibles, beverages, health care products, and medicines. The rapid detection and differentiation of phytocannabinoids is, therefore, essential to assess the potency and the therapeutic and nutritional values of cannabis cultivars. Here, we implemented SpiderMass technology for in vivo detection of cannabidiolic acid (CBDA) and ∆-tetrahydrocannabinolicacid (∆-THCA), and other endogenous organic plant compounds, to access distribution gradients within the plants and differentiate between cultivars. The SpiderMass system is composed of an IR-laser handheld microsampling probe connected to a mass spectrometer through a transfer tube. The analysis was performed on different plant organs from freshly cultivated cannabis plants in only a few seconds. SpiderMass analysis easily discriminated the two acid phytocannabinoid isomers via MS/MS, and the built statistical models differentiated between four cannabis cultivars. Different abundancies of the two acid phytocannabinoids were found along the plant as well as between different cultivars. Overall, these results introduce direct analysis by SpiderMass as a compelling analytical alternative for rapid hemp analysis.
近年来，大麻和基于大麻的产品在娱乐用途、食品、饮料、保健品和药品方面越来越受欢迎。因此，对植物大麻素进行快速检测和区分对于评估大麻品种的效力以及治疗和营养价值至关重要。在此，我们采用SpiderMass技术对大麻二酚酸（CBDA）和Δ-四氢大麻酚酸（Δ-THCA）以及其他内源性有机植物化合物进行体内检测，以了解植物体内的分布梯度并区分不同品种。SpiderMass系统由一个通过传输管连接到质谱仪的红外激光手持式微量采样探头组成。仅需几秒钟就能对新鲜种植的大麻植物的不同植物器官进行分析。SpiderMass分析通过串联质谱轻松区分了两种酸性植物大麻素异构体，并且所建立的统计模型区分了四个大麻品种。在不同品种之间以及沿植物分布发现了两种酸性植物大麻素的不同丰度。总体而言，这些结果表明SpiderMass直接分析是一种用于快速大麻分析的极具吸引力的分析方法。
