Kim Kyeong-Seog, Lee Yujin, Chae Woori, Cho Joo-Youn
Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital, Seoul 03080, Korea.
Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea.
Metabolites. 2022 Jun 7;12(6):525. doi: 10.3390/metabo12060525.
Gut microbial metabolites, short-chain fatty acids (SCFAs), are found at multiple locations in the host body and are identified as important metabolites in gut microbiome-associated diseases. Quantifying SCFAs in diverse biological samples is important to understand their roles in host health. This study developed an accurate SCFA quantification method by performing gas chromatography-mass spectrometry (GC/MS) in human plasma, serum, feces, and mouse cecum tissue. The samples were acidified with hydrochloric acid, and the SCFAs were extracted using methyl tert-butyl ether. In this method, distilled water was selected as a surrogate matrix for the quantification of SCFAs in target biological samples. The method was validated in terms of linearity, parallelism, precision, recovery, and matrix effect. The developed method was further applied in target biological samples. In conclusion, this optimized method can be used as a simultaneous SCFA quantification method in diverse biological samples.
肠道微生物代谢产物短链脂肪酸（SCFAs）在宿主体内的多个部位被发现，并被确定为肠道微生物群相关疾病中的重要代谢产物。对不同生物样品中的SCFAs进行定量分析，对于了解它们在宿主健康中的作用至关重要。本研究通过在人血浆、血清、粪便和小鼠盲肠组织中进行气相色谱-质谱联用（GC/MS），开发了一种准确的SCFAs定量方法。样品用盐酸酸化，并用甲基叔丁基醚提取SCFAs。在该方法中，选择蒸馏水作为替代基质，用于定量目标生物样品中的SCFAs。该方法在线性、平行性、精密度、回收率和基质效应方面进行了验证。所开发的方法进一步应用于目标生物样品。总之，这种优化后的方法可作为一种同时对不同生物样品中的SCFAs进行定量的方法。
