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来自柳属植物的乙酰辅酶A C-乙酰转移酶基因（）的分子克隆与分析

Molecular Cloning and Analysis of an Acetyl-CoA C-acetyltransferase Gene () from Liou.

作者信息

Wang Meng, Zheng Zhe, Tian Zheni, Zhang Hao, Zhu Chenyu, Yao Xiangyu, Yang Yixin, Cai Xia

机构信息

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China.

Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China.

出版信息

Plants (Basel). 2022 Jun 9;11(12):1539. doi: 10.3390/plants11121539.

DOI:10.3390/plants11121539
PMID:35736690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9229008/
Abstract

Terpenoids are the largest class of natural products and are essential for cell functions in plants and their interactions with the environment. Acetyl-CoA acetyltransferase (AACT, EC2.3.1.9) can catalyze a key initiation step of the mevalonate pathway (MVA) for terpenoid biosynthesis and is modulated by many endogenous and external stimuli. Here, the function and expression regulation activities of AACT in Liou (EkAACT) were reported. Compared with wild-type Arabidopsis, the root length, whole seedling fresh weight and growth morphology of -overexpressing plants were slightly improved. The transcription levels of , , , , and in the MVA pathway and total triterpenoid accumulation increased significantly in transgenic Arabidopsis. Under NaCl and PEG treatment, -overexpressing Arabidopsis showed a higher accumulation of total triterpenoids, higher enzyme activity of peroxidase (POD) and superoxide dismutase (SOD), increased root length and whole seedling fresh weight, and a decrease in the proline content, which indicated that plant tolerance to abiotic stress was enhanced. Thus, AACT, as the first crucial enzyme, plays a major role in the overall regulation of the MVA pathway.

摘要

萜类化合物是最大的一类天然产物，对植物的细胞功能及其与环境的相互作用至关重要。乙酰辅酶A乙酰转移酶（AACT，EC2.3.1.9）可催化萜类生物合成甲羟戊酸途径（MVA）的关键起始步骤，并受到许多内源性和外源性刺激的调节。本文报道了柳属植物（EkAACT）中AACT的功能及表达调控活性。与野生型拟南芥相比，过表达植株的根长、全株鲜重和生长形态略有改善。转基因拟南芥中MVA途径中相关基因的转录水平以及总三萜积累量显著增加。在NaCl和PEG处理下，过表达拟南芥表现出总三萜积累量更高、过氧化物酶（POD）和超氧化物歧化酶（SOD）的酶活性更高、根长增加、全株鲜重增加以及脯氨酸含量降低，这表明植物对非生物胁迫的耐受性增强。因此，AACT作为第一个关键酶，在MVA途径的整体调控中起主要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71a7/9229008/34c209a8b29b/plants-11-01539-g012.jpg
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3
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4
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