Pozharskiy Alexandr, Kostyukova Valeriya, Nizamdinova Gulnaz, Kalendar Ruslan, Gritsenko Dilyara
Laboratory of Molecular Biology, Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.
Department of Molecular Biology and Genetics, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Plants (Basel). 2022 Jun 16;11(12):1588. doi: 10.3390/plants11121588.
MLO proteins are a family of transmembrane proteins in land plants that play an important role in plant immunity and host-pathogen interactions, as well as a wide range of development processes. Understanding the evolutionary history of MLO proteins is important for understanding plant physiology and health. In the present work, we conducted a phylogenetic analysis on a large set of MLO protein sequences from publicly available databases, specifically emphasising MLOs from the tomato plant and related species. As a result, 4886 protein sequences were identified and used to construct a phylogenetic tree. In comparison to previous findings, we identified nine phylogenetic clades, revealed the internal structure of clades I and II as additional clades and showed the presence of monocotyledon species in all MLO clades. We identified a set of 19 protein motifs that allowed for the identification of particular clades. Sixteen SlMLO proteins from tomato were located in the phylogenetic tree and identified in relation to homologous sequences from other Solanaceae species. The obtained results could be useful for further work on the use of MLO proteins in the study of mildew resistance in Solanaceae and other plant families.
MLO蛋白是陆生植物中的一类跨膜蛋白，在植物免疫、宿主-病原体相互作用以及广泛的发育过程中发挥重要作用。了解MLO蛋白的进化历史对于理解植物生理学和健康状况至关重要。在本研究中，我们对来自公开数据库的大量MLO蛋白序列进行了系统发育分析，特别着重于番茄植株及相关物种的MLO蛋白。结果，我们鉴定出4886个蛋白序列，并用于构建系统发育树。与先前的研究结果相比，我们识别出九个系统发育分支，揭示了分支I和II的内部结构为额外的分支，并表明所有MLO分支中均存在单子叶植物物种。我们鉴定出一组19个蛋白基序，可用于识别特定的分支。番茄中的16个SlMLO蛋白定位在系统发育树中，并与其他茄科物种的同源序列相关联进行了鉴定。所获得的结果可能有助于在茄科和其他植物科的抗白粉病研究中进一步利用MLO蛋白开展工作。
