Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
Toxins (Basel). 2022 Jun 8;14(6):395. doi: 10.3390/toxins14060395.
Fungi belonging to the genus are frequently detected in water-damaged indoor environments, and a potential correlation between emerging health problems of inhabitants of affected housing and the fungi is controversially discussed. Secondary metabolites (i.e., mycotoxins) produced by , such as the highly toxic macrocyclic trichothecenes (MCTs), are of potential concern to human health. The present study, however, focused on the potential effects of the more broadly and abundantly formed group of phenylspirodrimanes (PSDs). The phase I and II metabolism of four structurally different PSDs were investigated in vitro using hepatic models in combination with high-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS) analysis. In addition to metabolite detection by HRMS, isolation and structure elucidation by nuclear magnetic resonance spectroscopy (NMR) was part of the conducted study as well.
属于 属的真菌经常在受潮的室内环境中被检测到，并且居住在受影响房屋中的居民出现健康问题与真菌之间的潜在相关性存在争议。 属产生的次生代谢产物（例如，霉菌毒素），如高毒性的大环三萜烯（MCT），对人类健康具有潜在的关注。然而，本研究主要集中在更广泛和丰富形成的苯并螺二氢吡喃烷（PSD）组的潜在影响上。使用肝模型结合高效液相色谱高分辨率质谱（HPLC-HRMS）分析，体外研究了四种结构不同的 PSD 的 I 期和 II 期代谢。通过 HRMS 进行代谢物检测，通过核磁共振波谱（NMR）进行分离和结构阐明也是进行研究的一部分。
