Romer Labs Diagnostic GmbH, Analytical Service Department, Technopark 5, 3430 Tulln, Austria.
Romer Labs Division Holding GmbH, Marketing Department, Erber Campus 1, 3131 Getzersdorf, Austria.
Toxins (Basel). 2022 Jun 13;14(6):405. doi: 10.3390/toxins14060405.
The present interlaboratory comparison study involved nine laboratories located throughout the world that tested for 24 regulated and non-regulated mycotoxins by applying their in-house LC-MS/MS multi-toxin method to 10 individual lots of 4 matrix commodities, including complex chicken and swine feed, soy and corn gluten. In total, more than 6000 data points were collected and analyzed statistically by calculating a consensus value in combination with a target standard deviation following a modified Horwitz equation. The performance of each participant was evaluated by a z-score assessment with a satisfying range of ±2, leading to an overall success rate of 70% for all tested compounds. Equal performance for both regulated and emerging mycotoxins indicates that participating routine laboratories have successfully expanded their analytical portfolio in view of potentially new regulations. In addition, the study design proved to be fit for the purpose of providing future certified reference materials, which surpass current analyte matrix combinations and exceed the typical scope of the regulatory framework.
本实验室间比对研究涉及全球 9 家实验室，它们通过应用各自的 LC-MS/MS 多毒素方法，对包括复杂的鸡和猪饲料、大豆和玉米面筋在内的 4 种基质商品的 10 个单独批次进行了 24 种规定和非规定霉菌毒素的检测。总共收集了超过 6000 个数据点，并通过按照改进的 Horwitz 方程计算共识值和目标标准偏差相结合的方式进行了统计分析。通过 z 分数评估对每个参与者的表现进行评估，范围在±2 之间令人满意，这导致所有测试化合物的总体成功率为 70%。对于所有受监管和新兴霉菌毒素的同等性能表明，参与的常规实验室已经成功扩展了他们的分析组合，以应对潜在的新法规。此外，研究设计被证明适合提供未来的认证参考材料，这些材料超越了当前的分析物基质组合，并超出了监管框架的典型范围。
