Chemistry Department, Oakland University, Rochester, Michigan 48309, United States.
Department of Chemistry, Xavier University of Louisiana, New Orleans, Louisiana 70125, United States.
Anal Chem. 2022 Jul 5;94(26):9242-9251. doi: 10.1021/acs.analchem.2c00017. Epub 2022 Jun 23.
Rapid and effective differentiation and quantification of a small molecule drug, such as fentanyl, in bodily fluids are major challenges for diagnosis and personal medication. However, the current toxicology methods used to measure drug concentration and metabolites require laboratory-based testing, which is not an efficient or cost-effective way to treat patients in a timely manner. Here, we show an assay for monitoring fentanyl levels by combining the intermolecular interaction-enabled small molecule recognition (iMSR) with differential impedance analysis of conjugated polymers. The differential interactions with the designed anchor interface were transduced through the perturbance of the electric status of the flexible conducting polymer. This assay showed excellent fentanyl selectivity against common interferences, as well as in variable body fluids through either testing strips or skin patches. Directly using the patient blood, the sensor provided 1%-5% of the average deviation compared to the "gold" standard method LC-MS results in the medically relevant fentanyl range of 20-90 nM. The superior sensing properties, in conjunction with mechanical flexibility and compatibility, enabled point-of-care detection and provided a promising avenue for applications beyond the scope of biomarker detection.
快速有效地鉴别和定量检测小分子药物，如芬太尼，在体液中是诊断和个人用药的主要挑战。然而，目前用于测量药物浓度和代谢物的毒理学方法需要基于实验室的检测，这不是一种及时有效地治疗患者的方法。在这里，我们展示了一种通过将分子间相互作用使小分子识别（iMSR）与共轭聚合物的差分阻抗分析相结合来监测芬太尼水平的分析方法。通过对柔性导电聚合物的电状态的扰动，将与设计的锚定界面的差分相互作用进行转换。该分析方法在测试条或贴片通过各种体液检测时，对设计的锚定界面表现出了对常见干扰物的优异芬太尼选择性，以及在可变体液中的优异选择性。该传感器直接使用患者血液，与“金”标准 LC-MS 方法相比，在 20-90 nM 的医学相关芬太尼范围内，其检测结果的平均偏差为 1%-5%。优异的传感性能，结合机械柔韧性和兼容性，实现了即时检测，并为超越生物标志物检测范围的应用提供了有前景的途径。
