Department of Physiology, Faculty of Medicine, Erzincan Binali Yildirim University, 24100, Erzincan, Turkey.
Department of Science Education, Education Faculty, Erciyes University, 38039, Kayseri, Turkey.
Sci Rep. 2022 Jun 23;12(1):10686. doi: 10.1038/s41598-022-15051-7.
Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite effective in anticancer treatments, their use in a wide spectrum and effective treatment possibilities are limited due to their systemic side effects and drug resistance development. In recent years, studies have focused on increasing the therapeutic efficacy of platinum-based drugs with drug delivery systems. Gelatin, a protein, obtained by the hydrolysis of collagen, is a biocompatible and biodegradable material that can be used in nano drug delivery systems. In this study, CP-loaded gelatin-based NPs (CP-NPs) were exposed to IR light in different temperatures at 30, 35, 40, 45, and 50 °C and characterized by FESEM-EDX, FTIR, UV-Vis, DLS. Accordingly, we synthesized gelatin-based CP-NPs of different sizes between 10-290 nm by exposure to IR. We found that CP-NPs-50, 16 nm nano-sized, obtained at 50 °C had the most cytotoxicity and was 2.2 times more effective than the free drug in HCT 116 colon cancer cells. Moreover, we showed that the cytotoxicity of CP-NPs-50 in normal HUVEC cells was lower. Additionally, we demonstrated that CP-NPs enhanced apoptotic activity while not developing MDR1-related resistance in colon cancer cells. In this study, for the first time drug loaded gelatin-based nanoparticles were synthesized in different sizes with a newly self-assembly method by exposing them to infrared light at different temperatures and their anticancer effects were evaluated subsequently.
卡铂（CP）是一种铂类似物，是治疗结直肠癌最广泛使用的化疗药物之一。虽然基于铂的药物在抗癌治疗中非常有效，但由于其全身副作用和耐药性的发展，其在广泛谱和有效治疗中的应用受到限制。近年来，研究集中在通过药物传递系统来提高基于铂的药物的治疗效果。明胶是一种由胶原蛋白水解得到的蛋白质，是一种生物相容性和可生物降解的材料，可用于纳米药物传递系统。在本研究中，CP 负载的明胶基 NPs（CP-NPs）在 30、35、40、45 和 50°C 的不同温度下暴露于 IR 光下，并通过 FESEM-EDX、FTIR、UV-Vis、DLS 进行了表征。因此，我们通过暴露于 IR 合成了不同大小的 10-290nm 之间的基于明胶的 CP-NPs。我们发现，在 50°C 下获得的 16nm 纳米大小的 CP-NPs-50 具有最强的细胞毒性，在 HCT 116 结肠癌细胞中的效力是游离药物的 2.2 倍。此外，我们表明 CP-NPs-50 在正常 HUVEC 细胞中的细胞毒性较低。此外，我们证明 CP-NPs 增强了细胞凋亡活性，而在结肠癌细胞中没有发展出 MDR1 相关的耐药性。在本研究中，首次通过在不同温度下暴露于红外光以新的自组装方法合成了不同大小的负载药物的明胶基纳米粒子，并随后评估了它们的抗癌作用。
