Makharza Sami, Vittorio Orazio, Cirillo Giuseppe, Oswald Steffen, Hinde Elizabeth, Kavallaris Maria, Büchner Bernd, Mertig Michael, Hampel Silke
Leibniz Institute for Solid State and Materials Research Dresden, 01171, Dresden, Germany,
Pharm Res. 2015 Jun;32(6):2132-43. doi: 10.1007/s11095-014-1604-z. Epub 2014 Dec 24.
Preparation of Nanographene oxide (NGO) - Gelatin hybrids for efficient treatment of Neuroblastoma.
Nanohybrids were prepared via non-covalent interactions. Spectroscopic tools have been used to discriminate the chemical states of NGO prior and after gelatin coating, with UV visible spectroscopy revealing the maximum binding capacity of gelatin to NGO. Raman and X-ray photoelectron spectroscopy (XPS) demonstrated NGO and Gelatin_NGO nanohybrids through a new chemical environments produced after noncovalent interaction. Microscopic analyses, atomic force microscopy (AFM) and scanning electron microscopy (SEM) are used to estimate the thickness of samples and the lateral width in the nanoscale, respectively.
The cell viability assay validated Gelatin_NGO nanohybrids as a useful nanocarrier for Carboplatin (CP) release and delivery, without obvious signs of toxicity. The nano-sized NGO (200 nm and 300 nm) did not enable CP to kill the cancer cells efficiently, whilst the CP loaded Gel_NGO 100 nm resulted in a synergistic activity through increasing the local concentration of CP inside the cancer cells.
The nanohybrids provoked high stability and dispersibility in physiological media, as well as enhanced the anticancer activity of the chemotherapy agent Carboplatin (CP) in human neuroblastoma cells.
制备用于高效治疗神经母细胞瘤的纳米氧化石墨烯（NGO）-明胶杂化物。
通过非共价相互作用制备纳米杂化物。使用光谱工具来区分明胶包覆前后NGO的化学状态，紫外可见光谱揭示了明胶与NGO的最大结合能力。拉曼光谱和X射线光电子能谱（XPS）通过非共价相互作用后产生的新化学环境证明了NGO和明胶-NGO纳米杂化物。微观分析，原子力显微镜（AFM）和扫描电子显微镜（SEM）分别用于估计样品的厚度和纳米尺度的横向宽度。
细胞活力测定验证了明胶-NGO纳米杂化物作为卡铂（CP）释放和递送的有用纳米载体，没有明显的毒性迹象。纳米尺寸的NGO（200nm和300nm）不能使CP有效地杀死癌细胞，而负载CP的100nm Gel_NGO通过增加癌细胞内CP的局部浓度产生协同活性。
纳米杂化物在生理介质中具有高稳定性和分散性，并增强了化疗药物卡铂（CP）在人神经母细胞瘤细胞中的抗癌活性。
