Arfuso Francesca, Rizzo Maria, Giannetto Claudia, Giudice Elisabetta, Cirincione Roberta, Cassata Giovanni, Cicero Luca, Piccione Giuseppe
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.
Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.
Antioxidants (Basel). 2022 Jun 15;11(6):1176. doi: 10.3390/antiox11061176.
This study aimed to evaluate the changes in serum oxidant and antioxidant parameters together with the serum values of homocysteine (Hcy) and muscle enzymes including creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in regularly trained athletic horses during official races of 1200, 1600 and 2000 m. Thirty Thoroughbred horses were divided into three groups of 10 subjects each according to the race distance: Group 1, 1200 m race; Group 2, 1600 m race; Group 3, 2000 m race. Blood samples were collected from horses 1 week prior to the race (1WB), on the day of the race at rest (TREST), immediately after the race (TPOST), and after 30 (TPOST30) and 120 (TPOST120) minutes. Serum total proteins, reactive oxygen metabolites (dROMs), thiol antioxidant barrier (SHp), antioxidant barrier (Oxy-ads), Hcy, CK, AST and LDH values were assessed. A two-way repeated measures ANOVA did not show differences referable to the race distance (Group effect) on all investigated parameters (p > 0.05). An exercise effect on oxidative stress markers, Hcy and muscle enzymes herein investigated was found in all groups (p < 0.001). A Pearson’s test showed dROMs positively correlated with SHp, Oxy-ads and Hcy after exercise (p < 0.05). This study suggests that, though well-trained racing horses are subjected to oxidative stress during a race, a proper antioxidant capacity may improve their ability to cope with exercise-induced oxidative stress.
本研究旨在评估在1200米、1600米和2000米正式比赛期间，经过定期训练的运动马匹血清中的氧化剂和抗氧化剂参数变化，以及同型半胱氨酸（Hcy）和肌肉酶（包括肌酸激酶（CK）、天冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH））的血清值。30匹纯种马根据比赛距离分为三组，每组10匹：第1组，1200米比赛；第2组，1600米比赛；第3组，2000米比赛。在比赛前1周（1WB）、比赛当天休息时（TREST）、比赛结束后立即（TPOST）、以及30分钟（TPOST30）和120分钟（TPOST120）后采集马匹的血样。评估血清总蛋白、活性氧代谢产物（dROMs）、硫醇抗氧化屏障（SHp）、抗氧化屏障（Oxy-ads）、Hcy、CK、AST和LDH值。双向重复测量方差分析显示，在所有研究参数上，比赛距离（组效应）没有差异（p>0.05）。在所有组中均发现运动对本文研究的氧化应激标志物、Hcy和肌肉酶有影响（p<0.001）。Pearson检验显示，运动后dROMs与SHp、Oxy-ads和Hcy呈正相关（p<0.05）。本研究表明，尽管训练有素的赛马在比赛中会受到氧化应激，但适当的抗氧化能力可能会提高它们应对运动诱导的氧化应激的能力。
