Shi Qingyu, Guo Yan, Yang Yang, Wu Shi, Han Renru, Ding Li, Yin Dandan, Hu Fupin
Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.
Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, China.
Antibiotics (Basel). 2022 May 30;11(6):737. doi: 10.3390/antibiotics11060737.
With the wide use of carbapenems, carbapenem-resistant have been increasingly reported worldwide. In this study, one -positive strain was isolated from the blood culture of a patient with a bloodstream infection in China, which was its first clinical report outside Pakistan. Species identification of was initially performed using MALDI-TOF/MS and further confirmed by 16S rRNA gene and housekeeping gene sequencing. The antimicrobial susceptibility testing was determined through the broth microdilution method, and their clonal relationship was analyzed by pulsed-field gel electrophoresis. To study the transmission and genetic structure of the gene, a transformation test and whole-genome sequencing (WGS) were performed. The results of the antimicrobial susceptibility testing indicated this was resistant to carbapenems, quinolones, and commonly used β-lactam/β-lactamase inhibitor combinations. Through WGS and transformation experiments, and genes causing antibiotic resistance were located on a 55,148-bp length IncX3 type plasmid with a truncated ColKp3 replicon gene. As a rare species of , was clinically reported in China for the first time, and the gene it carried was located on a globally disseminated IncX3 plasmid. The spread of such bacteria and antibiotic resistance requires more clinical attention.
随着碳青霉烯类药物的广泛使用，耐碳青霉烯类细菌在全球范围内的报道日益增多。在本研究中，从中国一名血流感染患者的血培养物中分离出一株阳性菌株，这是其在巴基斯坦境外的首次临床报道。最初使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）对该菌株进行菌种鉴定，并通过16S rRNA基因和管家基因测序进一步确认。通过肉汤微量稀释法进行抗菌药物敏感性试验，并通过脉冲场凝胶电泳分析它们的克隆关系。为了研究该基因的传播和遗传结构，进行了转化试验和全基因组测序（WGS）。抗菌药物敏感性试验结果表明，该菌株对碳青霉烯类、喹诺酮类以及常用的β-内酰胺/β-内酰胺酶抑制剂组合耐药。通过WGS和转化实验，导致抗生素耐药的基因位于一个长度为55,148 bp的IncX3型质粒上，该质粒带有一个截短的ColKp3复制子基因。作为一种罕见的菌株，该菌株在中国首次被临床报道，并且它携带的基因位于一个全球传播的IncX3质粒上。此类细菌及其抗生素耐药性的传播需要更多的临床关注。
