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MEG3 lncRNA 与活细胞中核斑点的关联。

The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells.

机构信息

The Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel.

出版信息

Cells. 2022 Jun 16;11(12):1942. doi: 10.3390/cells11121942.

DOI:10.3390/cells11121942
PMID:35741072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9221825/
Abstract

Nuclear speckles are nuclear bodies containing RNA-binding proteins as well as RNAs including long non-coding RNAs (lncRNAs). Maternally expressed gene 3 (MEG3) is a nuclear retained lncRNA found to associate with nuclear speckles. To understand the association dynamics of MEG3 lncRNA with nuclear speckles in living cells, we generated a fluorescently tagged MEG3 transcript that could be detected in real time. Under regular conditions, transient association of MEG3 with nuclear speckles was observed, including a nucleoplasmic fraction. Transcription or splicing inactivation conditions, known to affect nuclear speckle structure, showed prominent and increased association of MEG3 lncRNA with the nuclear speckles, specifically forming a ring-like structure around the nuclear speckles. This contrasted with metastasis-associated lung adenocarcinoma (MALAT1) lncRNA that is normally highly associated with nuclear speckles, which was released and dispersed in the nucleoplasm. Under normal conditions, MEG3 dynamically associated with the periphery of the nuclear speckles, but under transcription or splicing inhibition, MEG3 could also enter the center of the nuclear speckle. Altogether, using live-cell imaging approaches, we find that MEG3 lncRNA is a transient resident of nuclear speckles and that its association with this nuclear body is modulated by the levels of transcription and splicing activities in the cell.

摘要

核斑点是包含 RNA 结合蛋白以及包括长非编码 RNA（lncRNA）在内的 RNA 的核体。母系表达基因 3（MEG3）是一种与核斑点相关的核保留 lncRNA。为了了解 MEG3 lncRNA 在活细胞中与核斑点的关联动态，我们生成了一种可实时检测的荧光标记 MEG3 转录本。在常规条件下，观察到 MEG3 与核斑点的短暂关联，包括核质部分。转录或剪接失活条件会影响核斑点结构，导致 MEG3 lncRNA 与核斑点的显著和增加的关联，特别是在核斑点周围形成环状结构。这与通常与核斑点高度相关的转移性肺腺癌（MALAT1）lncRNA 形成对比，后者在核质中释放和分散。在正常条件下，MEG3 与核斑点的外围动态关联，但在转录或剪接抑制下，MEG3 也可以进入核斑点的中心。总之，通过活细胞成像方法，我们发现 MEG3 lncRNA 是核斑点的短暂驻留物，其与该核体的关联受细胞中转录和剪接活性水平的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/4ce25ae88da4/cells-11-01942-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/d635c25aa2cb/cells-11-01942-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/5b3cfcd6e900/cells-11-01942-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/8f7afdd41dd0/cells-11-01942-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/00284558d053/cells-11-01942-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/9d48c5a14494/cells-11-01942-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/110d23265aae/cells-11-01942-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/fac409ea3114/cells-11-01942-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/4ce25ae88da4/cells-11-01942-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/d635c25aa2cb/cells-11-01942-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/5b3cfcd6e900/cells-11-01942-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/8f7afdd41dd0/cells-11-01942-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/00284558d053/cells-11-01942-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/9d48c5a14494/cells-11-01942-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/110d23265aae/cells-11-01942-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/fac409ea3114/cells-11-01942-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d8/9221825/4ce25ae88da4/cells-11-01942-g008.jpg

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