Oliveira Neto M P, Grimaldi G, Momen H, Pacheco R S, Marzochi M C, McMahon Pratt D
Mem Inst Oswaldo Cruz. 1986 Jul-Sep;81(3):303-9. doi: 10.1590/s0074-02761986000300006.
L.d. chagasi was isolated from active cutaneous leishmaniasis in both human and canine infections in an endemic area in Rio de Janeiro, Brazil. Both isolates were identified by molecular and immunological characterization of the parasite using three different methods: electrophoretic mobility of isoenzymes; restriction endonuclease fragment analysis of kDNA and serodeme analysis using monoclonal antibodies. This seems to be the first well documented case in the New World of a "viscerotropic" Leishmania inducing a case of cutaneous leishmaniasis. This observation emphasizes that the diagnosis of the etiologic agent of human or canine visceral leishmaniasis based solely upon clinical and epidemiological criteria may lead to erroneous conclusions.
在巴西里约热内卢的一个流行地区，从人类和犬类感染的活动性皮肤利什曼病中分离出了恰加斯利什曼原虫。使用三种不同方法对寄生虫进行分子和免疫学特征鉴定，从而确定了这两种分离株：同工酶的电泳迁移率；kDNA的限制性内切酶片段分析以及使用单克隆抗体的血清型分析。这似乎是新世界中首例有充分记录的“亲内脏性”利什曼原虫引发皮肤利什曼病的病例。这一观察结果强调，仅基于临床和流行病学标准诊断人类或犬类内脏利什曼病的病原体可能会得出错误结论。
